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29176
CD68 (E3O7V) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

CD68 (E3O7V) Rabbit mAb (BSA and Azide Free) #29176

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Filter:
  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: CD68 (E3O7V) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various mouse cells using CD68 (E3O7V) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 2: CD68 (E3O7V) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse CD68 protein (mCD68-Myc/DDK; +), using CD68 (E3O7V) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CD68 (E3O7V) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mammary tumor using CD68 (E3O7V) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: CD68 (E3O7V) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using CD68 (E3O7V) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: CD68 (E3O7V) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using CD68 (E3O7V) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: CD68 (E3O7V) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse liver using CD68 (E3O7V) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: CD68 (E3O7V) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD68 (E3O7V) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: CD68 (E3O7V) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded RAW 264.7 cell pellet (left, positive), Renca cell pellet (middle, positive), or Neuro-2a cell pellet (right, negative) using CD68 (E3O7V) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: CD68 (E3O7V) Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse colon (left) and liver (right) using CD68 (E3O7V) Rabbit mAb (red). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) (Alexa Fluor® 488 Conjugate) #68206 (green). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: CD68 (E3O7V) Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of RAW 264.7 cells (left, positive) and Neuro-2a cells (right, negative) using CD68 (E3O7V) Rabbit mAb (green). Cells were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
To Purchase # 29176SF
Product # Size Price
29176SF
100 µg $ 746

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 70-80, 130-140, 200
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #97778. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #97778

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CD68 (E3O7V) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD68 protein. Staining of uncertain specificity in mouse endometrial epithelium has been observed by immunohistochemistry.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with mouse CD68 recombinant protein and reacts with an epitope surrounding Gly113.

Background

CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (1, 2). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (1-3). It is proposed to bind OxLDL and has been observed as a homodimer (3, 4).
  1. Rabinowitz, S.S. and Gordon, S. (1991) J Exp Med 174, 827-36.
  2. Holness, C.L. and Simmons, D.L. (1993) Blood 81, 1607-13.
  3. Ramprasad, M.P. et al. (1995) Proc Natl Acad Sci U S A 92, 9580-4.
  4. Ramprasad, M.P. et al. (1996) Proc Natl Acad Sci U S A 93, 14833-8.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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