CD68 (E3O7V) Rabbit mAb #97778
- WB
- IHC
- IF
- F
Supporting Data
| REACTIVITY | M R Hm |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70-80, 130-140, 200 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
- Hm-Hamster
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:600 - 1:2400 |
| Immunohistochemistry (Paraffin) | 1:150 - 1:600 |
| Immunofluorescence (Frozen) | 1:400 - 1:1600 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:200 |
| Flow Cytometry (Live) | 1:50 - 1:200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #29176.
Protocol
Specificity / Sensitivity
CD68 (E3O7V) Rabbit mAb recognizes endogenous levels of total CD68 protein. Staining of uncertain specificity in mouse endometrial epithelium has been observed by immunohistochemistry.
Species Reactivity:
Mouse, Rat, Hamster
Source / Purification
Monoclonal antibody is produced by immunizing animals with mouse CD68 recombinant protein and reacts with an epitope surrounding Gly113.
Background
CD68 (macrosialin) is a heavily glycosylated transmembrane protein that is expressed by and commonly used as a marker for monocytes and macrophages (1,2). It is found on the plasma membrane, as well as endosomal and lysosomal membranes (1-3). It is proposed to bind OxLDL and has been observed as a homodimer (3,4).
Limited Uses
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