CD79B (D7V2F) Rabbit mAb (BSA and Azide Free) #59728
- WB
- IHC
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 14, 30-50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
This product is the carrier free version of product #96024. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
CD79B (D7V2F) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD79B protein. This antibody is predicted to detect multiple isoforms of CD79B.
Species Reactivity:
Human, Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Rat, Hamster, Bovine, Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val222 of human CD79B protein.
Background
The B cell antigen receptor (BCR) is composed of membrane immunoglobulin molecules non-covalently associated with the heterodimeric signaling component, CD79A and CD79B (also known as Igα and Igβ, respectively) (1,2). The presence of this receptor complex is essential for B cell development and function (3). Following antigen binding, CD79A/CD79B heterodimers are phosphorylated and initiate intracellular signaling through Src family kinases, Lyn, Blk, and Fyn, as well as Syk and Btk tyrosine kinases (4,5). The complexity of BCR signaling results in a variety of distinct cellular functions, such as proliferation, tolerance, apoptosis, and differentiation (6). BCR-antigen ligation also leads to internalization of the complex, trafficking to late endosomes, and antigen presentation in major histocompatibility molecules on the B cell surface (7,8). CD79B enhances the phosphorylation of CD79A (9). Alternatively spliced transcript variants encoding different isoforms of CD79B have been identified (10). CD79B is widely expressed on B cell malignancies and may serve as a target for therapeutic intervention (11,12).
- Mason, D.Y. et al. (1995) Blood 86, 1453-9.
- Storch, B. et al. (2007) Eur J Immunol 37, 252-60.
- Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7.
- Reth, M. and Wienands, J. (1997) Annu Rev Immunol 15, 453-79.
- Kurosaki, T. and Kurosaki, M. (1997) J Biol Chem 272, 15595-8.
- Dal Porto, J.M. et al. (2004) Mol Immunol 41, 599-613.
- Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21.
- van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8.
- Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63.
- Hashimoto, S. et al. (1995) Mol Immunol 32, 651-9.
- Dornan, D. et al. (2009) Blood 114, 2721-9.
- Pfeifer, M. et al. (2015) Leukemia 29, 1578-86.
Limited Uses
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