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59728
CD79B (D7V2F) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

CD79B (D7V2F) Rabbit mAb (BSA and Azide Free) #59728

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  1. WB
  2. IHC
Western Blotting Image 1: CD79B (D7V2F) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines and tissues using CD79B (D7V2F) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CD79B (D7V2F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human tonsil using CD79B (D7V2F) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: CD79B (D7V2F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD79B (D7V2F) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: CD79B (D7V2F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD79B (D7V2F) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: CD79B (D7V2F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Daudi cell pellets (left, positive) and Jurkat cell pellets (right, negative) using CD79B (D7V2F) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 59728SF
Product # Size Price
59728SF
100 µg $ 746

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 14, 30-50
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #96024. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #96024.

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CD79B (D7V2F) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD79B protein. This antibody is predicted to detect multiple isoforms of CD79B.

Species Reactivity:

Human, Mouse

Species predicted to react based on 100% sequence homology:

Rat, Hamster, Bovine, Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val222 of human CD79B protein.

Background

The B cell antigen receptor (BCR) is composed of membrane immunoglobulin molecules non-covalently associated with the heterodimeric signaling component, CD79A and CD79B (also known as Igα and Igβ, respectively) (1,2). The presence of this receptor complex is essential for B cell development and function (3). Following antigen binding, CD79A/CD79B heterodimers are phosphorylated and initiate intracellular signaling through Src family kinases, Lyn, Blk, and Fyn, as well as Syk and Btk tyrosine kinases (4,5). The complexity of BCR signaling results in a variety of distinct cellular functions, such as proliferation, tolerance, apoptosis, and differentiation (6). BCR-antigen ligation also leads to internalization of the complex, trafficking to late endosomes, and antigen presentation in major histocompatibility molecules on the B cell surface (7,8). CD79B enhances the phosphorylation of CD79A (9). Alternatively spliced transcript variants encoding different isoforms of CD79B have been identified (10). CD79B is widely expressed on B cell malignancies and may serve as a target for therapeutic intervention (11,12).
  1. Mason, D.Y. et al. (1995) Blood 86, 1453-9.
  2. Storch, B. et al. (2007) Eur J Immunol 37, 252-60.
  3. Yu, L.M. and Chang, T.W. (1992) J Immunol 148, 633-7.
  4. Reth, M. and Wienands, J. (1997) Annu Rev Immunol 15, 453-79.
  5. Kurosaki, T. and Kurosaki, M. (1997) J Biol Chem 272, 15595-8.
  6. Dal Porto, J.M. et al. (2004) Mol Immunol 41, 599-613.
  7. Minegishi, Y. et al. (1999) J Clin Invest 104, 1115-21.
  8. van Noesel, C.J. et al. (1991) J Immunol 146, 3881-8.
  9. Luisiri, P. et al. (1996) J Biol Chem 271, 5158-63.
  10. Hashimoto, S. et al. (1995) Mol Immunol 32, 651-9.
  11. Dornan, D. et al. (2009) Blood 114, 2721-9.
  12. Pfeifer, M. et al. (2015) Leukemia 29, 1578-86.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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