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CD8 (RIV11) Mouse mAb #3572

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Inquiry Info. # 3572

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 32
    Source/Isotype Mouse IgG1
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:25

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD8 (RIV11) Mouse mAb detects endogenous levels of total CD8 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing BALB/c mice with human peripheral lymphocytes.

    Background

    Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

    Alternate Names

    CD8; CD8 antigen alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide (p37); CD8 antigen, beta polypeptide 1 (p37); CD8A; CD8a molecule; CD8B; CD8b antigen; CD8b molecule; CD8B1; Leu2; Leu2 T-lymphocyte antigen; Ly3; LYT3; MAL; MGC119115; OKT8 T-cell antigen; p32; P37; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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