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71381
CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

CD81 (D3N2D) Rabbit mAb (BSA and Azide Free) #71381

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  1. WB
  2. IHC
Western Blotting Image 1: CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using CD81 (D3N2D) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The absence of signal for CD81 in U-937 cells is consistent with published reports. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using CD81 (D3N2D) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using CD81 (D3N2D) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using CD81 (D3N2D) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using CD81 (D3N2D) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human mantle cell lymphoma using CD81 (D3N2D) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human liver using CD81 (D3N2D) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Small Intestine: Immunohistochemical analysis of paraffin-embedded normal human small intestine using CD81 (D3N2D) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human kidney using CD81 (D3N2D) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 9: CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human brain using CD81 (D3N2D) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 10: CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human liver using CD81 (D3N2D) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 11: CD81 (D3N2D) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded U-937 cell pellet (left, positive) or RL cell pellet (right, negative) using CD81 (D3N2D) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 71381
Cat. # Size Qty. Price
71381SF
100 µg
$ 758

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 22
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #56039. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #56039

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

CD81 (D3N2D) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD81 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile181 within an extracellular region of human CD81 protein.

Background

CD81 belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2) (1-3). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin enriched microdomains (TEMs) where they mediate a range of processes including adhesion, motility, membrane organization, and signal transduction (3). CD81, like other tetraspanins, is enriched in exosomes (4). Many research studies demonstrate a role for CD81 in lymphocyte signaling (5-7). CD81 is also a well-characterized receptor for Hepatitis C Virus and facilitates the entry of the virus into target cells (8,9).
  1. Oren, R. et al. (1990) Mol Cell Biol 10, 4007-15.
  2. Levy, S. et al. (1991) J Biol Chem 266, 14597-602.
  3. Hemler, M.E. (2005) Nat Rev Mol Cell Biol 6, 801-11.
  4. Escola, J.M. et al. (1998) J Biol Chem 273, 20121-7.
  5. Levy, S. et al. (1998) Annu Rev Immunol 16, 89-109.
  6. Levy, S. and Shoham, T. (2005) Nat Rev Immunol 5, 136-48.
  7. Jones, E.L. et al. (2011) Biochem Soc Trans 39, 506-11.
  8. Pileri, P. et al. (1998) Science 282, 938-41.
  9. Bartosch, B. et al. (2003) J Biol Chem 278, 41624-30.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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