Render Target: STATIC
Render Timestamp: 2024-10-08T10:15:28.233Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD83 (D8V7V) Rabbit mAb #99075

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 23-65
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD83 (D8V7V) Rabbit mAb recognizes endogenous levels of total CD83 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro63 of human CD83 protein.

    Background

    CD83 is a single-transmembrane protein with a calculated molecular weight (MW) of 23 kDa, but due to heavy and differential glycosylation, its apparent MW ranges from 23 to 70 kDa. CD83 is predominantly expressed on mature dendritic cells (DCs) and has been used as a DC activation/maturation marker as its increased expression is correlated with upregulation of HLA class II antigen expression on DCs (1-3). CD83 is also expressed at a low level on lymphocytes and is upregulated upon lymphocyte activation (4, 5). Thymic epithelial cells also express CD83, which is required for normal CD4+ T cell development (6, 7). CD83 is also expressed as a soluble form (sCD83) that can be found in serum of healthy adults (8). sCD83 has been shown to negatively regulate immune response by lymphocytes (9, 10).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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