CD84 (E4F2N) Rabbit mAb #80519
- WB
- IP
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45-80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Protocol
Specificity / Sensitivity
CD84 (E4F2N) Rabbit mAb recognizes endogenous levels of total CD84 protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse CD84 protein.
Background
Cluster of Differentiation 84 (CD84), also known as signaling lymphocytic activation molecule family member 5 (SLAMF5), is a membrane glycoprotein expressed by numerous immune cell types, including hematopoietic progenitor cells, T cells, B cells, NK cells, macrophages, monocytes, dendritic cells, platelets, mast cells, and thymocytes (1-9). CD84 is a homophilic adhesion molecule involved in modulating the activation, differentiation, proliferation, and cytotoxicity of a wide variety of immune cells, and thus is involved in the regulation and interconnection of both the innate and adaptive immune response (6-14). Certain CD84 activities are regulated by the presence or absence of small cytoplasmic adaptor proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (5,14). Alternate splicing results in multiple transcript variants.
- Zaiss, M. et al. (2003) Exp Hematol 31, 798-805.
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- Krause, S.W. et al. (2000) Biochem J 346 Pt 3, 729-36.
- Romero, X. et al. (2004) Tissue Antigens 64, 132-44.
- Tangye, S.G. et al. (2002) Eur J Immunol 32, 1640-9.
- Álvarez-Errico, D. et al. (2011) J Immunol 187, 5577-86.
- Nanda, N. et al. (2005) Blood 106, 3028-34.
- Tangye, S.G. et al. (2003) J Immunol 171, 2485-95.
- Martin, M. et al. (2001) J Immunol 167, 3668-76.
- Sintes, J. et al. (2010) J Leukoc Biol 88, 687-97.
- Wong, E.B. et al. (2015) J Immunol 194, 4130-43.
- Agod, Z. et al. (2018) Front Immunol 9, 62.
- Cannons, J.L. et al. (2010) Immunity 32, 253-65.
- Wang, N. et al. (2010) J Immunol 185, 5683-7.
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