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76755
CD86 (E2G8P) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

CD86 (E2G8P) Rabbit mAb (BSA and Azide Free) #76755

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Filter:
  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: CD86 (E2G8P) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using CD86 (E2G8P) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CD86 (E2G8P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using CD86 (E2G8P) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: CD86 (E2G8P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using CD86 (E2G8P) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: CD86 (E2G8P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded HDLM-2 cell pellet (left, positive) or HT-29 cell pellet (right, negative) using CD86 (E2G8P) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: CD86 (E2G8P) Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of HDLM-2 cells (left, positive) and HT-29 cells (right, negative) using CD86 (E2G8P) Rabbit mAb (green) and Hoechst 33342 #4082 (blue). Data were generated using the standard formulation of this product.
To Purchase # 76755
Cat. # Size Qty. Price
76755SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 60-85
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #91882. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #91882

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CD86 (E2G8P) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD86 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro239 of human CD86 protein.

Background

CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9).
  1. Freeman, G.J. et al. (1989) J Immunol 143, 2714-22.
  2. Azuma, M. et al. (1993) Nature 366, 76-9.
  3. Adams, A.B. et al. (2016) J Immunol 197, 2045-50.
  4. Linsley, P.S. et al. (1990) Proc Natl Acad Sci U S A 87, 5031-5.
  5. Gimmi, C.D. et al. (1991) Proc Natl Acad Sci U S A 88, 6575-9.
  6. Harding, F.A. et al. (1992) Nature 356, 607-9.
  7. Collins, A.V. et al. (2002) Immunity 17, 201-10.
  8. Linsley, P.S. et al. (1991) J Exp Med 174, 561-9.
  9. Krummel, M.F. and Allison, J.P. (1995) J Exp Med 182, 459-65.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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