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CD89/FCAR (F1T9J) Rabbit mAb (BSA and Azide Free)

CD89/FCAR (F1T9J) Rabbit mAb (BSA and Azide Free) #85114

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	60-80
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

This product is the carrier free version of product #26013. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na₂HPO₄, 3 mM KCl, 2 mM KH₂PO₄, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #26013

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

CD89/FCAR (F1T9J) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD89/FCAR protein. Non-specific staining was observed in pancreatic acinar cells and apical membrane of colon epithelium by immunohistochemistry.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD89/FCAR protein.

Background

Immunoglobulin alpha Fc (IgA Fc) receptor (FcαRI), also known as CD89/FCAR, is a member of the Fc receptor superfamily and is expressed as a transmembrane glycoprotein on the surface of immune cells of the myeloid lineage (1-3). Research studies have demonstrated that CD89/FCAR can bind to polymeric IgA-containing immune complexes, which mediates receptor activation, internalization, and signaling (4). Productive signaling by CD89/FCAR requires association with the FcR gamma-chain and subsequent engagement of the tyrosine kinases, Fyn and Syk, to drive activation of pro-inflammatory signaling cascades (1,5).

Database Links

UniProt ID: P24071

Entrez-Gene Id: 2204

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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