Ch-TOG (D2Z8J) Rabbit mAb #67774
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 225 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Ch-TOG (D2Z8J) Rabbit mAb recognizes endogenous levels of total ch-TOG protein. Based on the amino acid sequence of the peptide antigen, this antibody is expected to recognize all isoforms of ch-TOG.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala31 of human ch-TOG protein.
Background
Ch-TOG (colonic hepatic tumor overexpressed gene)/CKAP5 (cytoskeleton-associated protein 5) is a microtubule stabilizing protein involved in the organization of mitotic spindle poles through interaction with the transforming acid coiled-coil protein, TACC3 (1). Ch-TOG and TACC3 also interact with the membrane trafficking protein clathrin, and this interaction is thought to be required for clathrin’s mitotic function in crosslinking microtubules in the mitotic spindle (2). Researchers have found that expression levels of both TACC3 and ch-TOG are correlated with human diseases such as glioblastoma and hepatic carcinoma (3). A genome-wide siRNA screen identified ch-TOG and other G2/M phase regulators as potential contributors to head and neck squamous cell carcinoma (4).
Limited Uses
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