Render Target: STATIC
Render Timestamp: 2024-12-13T11:50:49.383Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-30 01:57:28.513
Product last modified at: 2024-11-21T15:30:11.486Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ChAT (E4F9G) Rabbit mAb #27269

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 71
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ChAT (E4F9G) Rabbit mAb recognizes endogenous levels of total human, mouse, and rat ChAT protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human ChAT protein.

    Background

    Choline O-acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from acetyl CoA and choline in the central and peripheral nervous system (1).  In mammals, ACh is the primary neurotransmitter used at the neuromuscular junction, released by motor neurons to activate muscles. Neurons that release ACh are known as cholinergic neurons, which express high levels of ChAT.  In addition to motor neurons, cholinergic neurons are present in the brain, particularly in the basal forebrain as well as other regions of the brain, including striatal interneurons. Reduction in ChAT, indicative of degeneration of cholinergic neurons, is correlated with cognitive decline in several neurodegenerative diseases, including Alzheimer’s disease (2). 
    For Research Use Only. Not For Use In Diagnostic Procedures.
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