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Render Timestamp: 2024-10-24T09:50:04.662Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-20 06:17:39.832
Product last modified at: 2024-09-05T12:00:28.613Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464
  1. Products /
  2. Primary Antibodies /
  3. ChREBP Antibody

ChREBP Antibody #58069

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 110
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ChREBP Antibody recognizes endogenous levels of total ChREBP protein. Based on amino acid sequence comparisons, the antibody is predicted to detect all known isoforms of ChREBP.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu308 of human ChREBP isoform α protein. The sequence surrounding this region is 100% conserved in all known isoforms of ChREBP. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Carbohydrate-responsive element-binding protein (ChREBP) is a glucose-responsive transcription factor that regulates glycolytic and lipogenic gene expression (1). High levels of glucose induce the transcriptional activity of ChREBP. ChREBP is most abundant in tissues of de novo lipogenesis and forms a heterotetramer with its binding partner MLX to bind to the promoter regions of its target genes (2). ChREBP regulates fatty acid synthesis, glucose homeostasis and insulin sensitivity in white adipose tissue. In white adipose tissue, ChREBP isoform α (ChREBP-α) activates the expression of the potent ChREBP isoform β (ChREBP-β) in a glucose-dependent manner. ChREBP-β expression levels predict insulin sensitivity in human white adipose tissue (1). In addition, research studies have shown that Akt2 induces the transcriptional activity of ChREBP-β to stimulate de novo lipogenesis in brown adipose tissue for fuel storage (3). Furthermore, ChREBP-β is a potent activator of lipogenesis in the liver (4).

    Database Links

    UniProt ID: Q9NP71


    Entrez-Gene Id: 51085


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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