Render Target: STATIC
Render Timestamp:
3/26/2025, 10:00:48 AM EDT
3/26/2025, 2:00:48 PM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:19:39.620
Product last modified at: 2025-01-01T09:02:45.258Z
Cell Signaling Technology Logo
View Featured Offers >>
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. CLCA1 (E4Y1J) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CLCA1 (E4Y1J) Rabbit mAb #77693

Filter:
  • WB
  • IP
  • IHC
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 45-60, 130
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:100 - 1:400
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CLCA1 (E4Y1J) Rabbit mAb recognizes endogenous levels of total CLCA1 protein. This antibody also recognizes the 45-60 kDa autoproteolytically cleaved form of CLCA1.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala745 of human CLCA1 protein.

    Background

    Many epithelial surfaces of the body are covered with protective mucus, and disrupted mucus homeostasis has been implicated in increased susceptibility to infection, along with diseases such as colitis, cystic fibrosis, chronic obstructive lung disease, asthma, and colon and lung cancers (1-4). The chloride channel calcium-activated (CLCA) family are secreted proteins that regulate chloride transport and mucin expression (2). CLCAs control the production of mucus in lung, colon, and other tissues (2,3). CLCA1, the first characterized human CLCA family member, is mainly expressed in the lungs and lower digestive tract, particularly intestinal crypt cells (1,2,6,7). CLCA1 has been linked to the pathogenesis of asthma and chronic obstructive pulmonary disease (5), in addition to colitis and colon cancer (1,3,6). CLCA1 expression is significantly altered in the tissues of these patients, and mutations in CLCA1 are associated with increased disease incidence (5,6). CLCA1 contains a consensus proteolytic cleavage site, centered at glycine 694, which is recognized by a metalloprotease domain located within its N terminus (2).

    Database Links

    UniProt ID: A8K7I4


    Entrez-Gene Id: 1179


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.