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Cleaved Caspase-8 (Asp374) Antibody (Human Specific) #9749

Inquiry Info. # 9749

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    Product Specifications

    REACTIVITY
    SENSITIVITY
    MW (kDa) 22
    SOURCE Rabbit

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Cleaved Caspase-8 (Asp374) Antibody (Human Specific) detects endogenous levels of the large fragment of caspase-8 resulting from cleavage at aspartic acid 374. This antibody does not cross-react with full length caspase-8.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp374 of human caspase-8. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

    Alternate Names

    ALPS2B; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; CASP-8; CASP8; caspase 8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; Caspase-8; Caspase-8 subunit p10; Caspase-8 subunit p18; caspase8; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE-like apoptotic protease 5; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MCH5; MGC78473; MORT1-associated ced-3 homolog

    For Research Use Only. Not for Use in Diagnostic Procedures.
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