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81375
COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free) #81375

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using COL1A1 (E8F4L) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human kidney using COL1A1 (E8F4L) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using COL1A1 (E8F4L) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse lung using COL1A1 (E8F4L) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse liver using COL1A1 (E8F4L) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using COL1A1 (E8F4L) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded GL-261 syngeneic tumor using COL1A1 (E8F4L) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma (left), renal cell carcinoma (middle) or prostate carcinoma (right) using COL1A1 (E8F4L) XP® Rabbit mAb (top) or COL1A1 (E3E1X) Mouse mAb #66948 (bottom). These two antibodies detect independent, unique epitopes on human COL1A1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using COL1A1 (E8F4L) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 9: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded U-118 MG cell pellet (left, positive) or HT-29 cell pellet (right, negative) using COL1A1 (E8F4L) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse spleen using COL1A1 (E8F4L) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #70034 (cyan pseudocolor) and α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #36110 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 2: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse liver using COL1A1 (E8F4L) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #70034 (cyan pseudocolor) and α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #36110 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 3: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse colon using COL1A1 (E8F4L) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP®Rabbit mAb (Alexa Fluor® 488 Conjugate) #70034 (cyan pseudocolor) and α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #36110 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of U-118 MG cells (left, positive) and HT-29 cells (right, negative) using COL1A1 (E8F4L) XP® Rabbit mAb (green) and DyLight 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
To Purchase # 81375SF
Product # Size Price
81375SF
100 µg $ 746

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 220
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #72026. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #72026

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

COL1A1 (E8F4L) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total COL1A1 protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe1197 of human COL1A1 protein.

Background

Type 1 collagen is the most abundant collagen in many human tissues, including bone, skin, and tendons. It is a trimeric complex comprised of two molecules of COL1A1 (alpha-1 type 1 collagen) and one molecule of COL1A2 (alpha-2 type 1 collagen) (1-3). The expression levels of COL1A1 are regulated by multiple mechanisms, including mRNA stability, translation, and post-translational modification (3-5). Overexpression of COL1A1 has been positively associated with tissue fibrosis disorders, including systemic sclerosis (6), while loss-of-function mutations in the COL1A1 gene are a major causative factor for osteogenesis imperfecta (brittle bone disease) (7). Notably, COL1A1 expression levels have also been associated with tumor development in gastric, lung, thyroid, and breast cancers. Research studies suggest that upregulation of COL1A1 can generate a modified extracellular matrix environment that promotes cancer cell survival, proliferation, metastasis, and invasion (8-11).
  1. Prockop, D.J. and Kivirikko, K.I. (1995) Annu Rev Biochem 64, 403-34.
  2. Chang, S.W. et al. (2012) Biophys J 102, 640-8.
  3. Zhang, Y. and Stefanovic, B. (2016) Int J Mol Sci 17, 419.
  4. Parsons, C.J. et al. (2011) J Biol Chem 286, 8609-19.
  5. Cai, L. et al. (2010) J Mol Biol 395, 309-26.
  6. Jimenez, S.A. and Saitta, B. (1999) Springer Semin Immunopathol 21, 397-414.
  7. Forlino, A. et al. (2011) Nat Rev Endocrinol 7, 540-57.
  8. Li, J. et al. (2016) World J Surg Oncol 14, 297.
  9. Oleksiewicz, U. et al. (2017) J Cancer Res Clin Oncol 143, 1133-41.
  10. Barcus, C.E. et al. (2017) Breast Cancer Res 19, 9.
  11. Jolly, L.A. et al. (2016) Cancer Res 76, 1804-13.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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