Render Target: STATIC
Render Timestamp: 2024-10-11T09:43:14.525Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:57:37.232
Product last modified at: 2024-09-30T08:00:47.544Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Complement C4-A/C4-B (E6J7W) Rabbit mAb #60059

Filter:
  • WB

    Supporting Data

    REACTIVITY H R
    SENSITIVITY Endogenous
    MW (kDa) 210
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Complement C4-A/C4-B (E6J7W) Rabbit mAb recognizes endogenous levels of total C4-A/C4-B protein. The predicted epitope of Complement C4-A/C4-B (E6J7W) Rabbit mAb is the N-terminus of the β-subunit of the C4-A/C4-B protein.

    Species Reactivity:

    Human, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro148 of human C4-A/C4-B protein.

    Background

    Complement is a collection of soluble proteins secreted in the blood and other body fluids (1). As part of the innate immune system, complement proteins are involved in the clearance of pathogens and damaged cells in a process called opsonization, which results in the coating of a pathogen with antibodies and/or complement proteins to facilitate phagocytosis of debris or foreign pathogens.  Complements are activated by a cascade of cleavage reactions, triggered initially by pattern recognition receptor-mediated detection of pathogens/debris. The cascade of cleavage-mediated products activate three distinct effector pathways, including inflammation, phagocytosis, and membrane attack that represent a coordinated defense of the host organism. Several complement proteins are encoded in the mammalian genome, designated by the capital letter C followed by a number, in order by their discovery. Complement C4-A and C4-B, encoded by the C4A and C4B genes, respectively, are the non-enzymatic components of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. The C4A/C4B genes generate C4-A/C4-B polypeptide proteins, composed of an alpha, beta, and gamma-chain subunits linked by disulfide bonds. The polypeptide protein can be further cleaved to generate a cascade of fragments to initiate the innate immune response.  In addition to the innate immune response, several components of the complement system, including C4-A, have been implicated in brain development, as well as neurodevelopmental and neurodegenerative diseases. The complement system plays a role in microglial-dependent synapse pruning of excess synapses during development, particularly in the visual system (2). Additionally, complement-mediated synaptic pruning may also contribute to psychiatric diseases such as schizophrenia (3,4).
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