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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Contactin-2 (D4M7G) Rabbit mAb #26372

Filter:
  • WB

    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 130
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Contactin-2 (D4M7G) Rabbit mAb recognizes endogenous levels of total contactin-2 protein.


    Species Reactivity:

    Mouse, Rat


    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val375 of human contactin-2 protein.

    Background

    Myelinated axons contain un-myelinated gaps called nodes of Ranvier. These regularly spaced gaps are critical for the proper propagation and rapid conduction of nerve impulses in the central and peripheral nervous system (1). The structure and organization of the nodes of Ranvier is dictated by interaction between the axon and glial cells (2). Voltage-gated sodium channels concentrated at the nodes and potassium channels clustered at the paranodes are responsible for propagation of the action potentials (3,4). Other proteins that contribute to the architecture and function of the nodes of Ranvier include βIV spectrin (5), ankyrin-G (6), and the L1 cell adhesion molecules, neurofascin and NrCAM (7,8).
    Contactin-2 (CNTN2, TAG-1) is a glycosyl-phosphatidyl-inositol-anchored cell adhesion protein that is expressed at the juxtaparanodal region of the nodes of Ranvier by oligodendrocytes, Schwann cells, and axons (9,10). Contactin-2 plays an important role in the proper organization of the juxtaparanodes through interaction with Caspr2 and the recruitment of the Kv1.1 and Kv1.2 potassium channel subunits (11). Research studies indicate that contactin-2 is a substrate of β-secretase 1 (12,13). A deletion mutation in the corresponding CNTN2 gene results in familial adult myoclonic epilepsy-5, which is characterized by seizures, involuntary myoclonic muscle twitches, and mild intellectual disability (14).

      For Research Use Only. Not For Use In Diagnostic Procedures.
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