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|H M R Mk||Endogenous||65||Rabbit|
Western blot analysis of extracts from various cell lines using CPEB1 Antibody.Learn more about how we get our images.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CPEB1 protein (hCPEB1-Myc/DDK), using CPEB1 Antibody (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).Learn more about how we get our images.
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
posted June 2005
revised June 2016
Protocol Id: 263
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
CPEB1 Antibody recognizes endogenous levels of total CPEB1 protein.
Human, Mouse, Rat, Monkey
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu170 of human CPEB1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Cytoplasmic polyadenylation element-binding protein (CPEB) is a conserved protein that binds RNA and regulates translation (1-3). CPEB binds to the cytoplasmic polyadenylation element (CPE) located in the 3′ untranslated region (UTR) of specific mRNAs expressed in germ cells, fibroblasts, and neurons (1-4). Together with other factors, CPEB promotes polyadenylation of mRNA transcripts to create mRNA poly(A) tails prior to translation (1-3). CPEB1 regulates meiotic and early-embryonic cell proliferation and is essential for mediation of poly(A) tail length changes required for mitotic cell division (4). Research studies suggest that CPEB1-mediated shortening of the 3'-UTR correlates with tumorigenesis (5).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. XP is a registered trademark of Cell Signaling Technology, Inc. Tween is a registered trademark of ICI Americas, Inc.
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