CXCL10 (D5L5L) Rabbit mAb #14969
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 10 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
CXCL10 (D5L5L) Rabbit mAb recognizes endogenous levels of total CXCL10 protein. This antibody also cross-reacts with a 100kDa protein of unknown orgin in some cell lines.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human CXCL10 protein.
Background
C-X-C motif chemokine ligand 10 (CXCL10, IP-10) is a chemokine produced by several cell types and induced in response to pro-inflammatory cytokines, such as IFN-γ and TNF-α (1,2). CXCL10 binds to the chemokine receptor CXCR3 and induces calcium flux and cell migration (3). The CXCR3 protein is normally expressed on activated CD4+ T cells and NK cells, which can produce pro-inflammatory cytokines, including IFN-γ and TNF-α (3,4). As a result, these molecules act in an amplification loop to promote immune responses in the contexts of both autoimmunity and a tumor immune response (5,6).
- Shin, S.Y. et al. (2010) J Biol Chem 285, 30731-40.
- Luster, A.D. and Ravetch, J.V. (1987) J Exp Med 166, 1084-97.
- Loetscher, M. et al. (1996) J Exp Med 184, 963-9.
- Kajitani, K. et al. (2012) Breast Cancer Res Treat 134, 139-55.
- Luster, A.D. and Leder, P. (1993) J Exp Med 178, 1057-65.
- Antonelli, A. et al. (2014) Autoimmun Rev 13, 272-80.
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