CXCR5 (D6L3C) Rabbit mAb #72172
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous (IHC-P) |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| IHC Leica Bond | 1:50 - 1:200 |
| Immunohistochemistry (Paraffin) | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier-free (BSA and azide free) version of this product see product #99825
Protocol
Specificity / Sensitivity
CXCR5 (D6L3C) Rabbit mAb recognizes endogenous levels of total CXCR5 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domains of human CXCR5 protein.
Background
CXCR5 is a G protein-coupled receptor belonging to the chemokine receptor subfamily (1). Upon binding of its ligand, the chemokine CXCL13, CXCR5 initiates multiple intracellular signaling pathways that regulate cell proliferation, survival, and migration (2). CXCR5 is expressed in both mature B cells and follicular helper T cells, and responds to CXCL13 gradient to control lymphocyte migration toward secondary lymphoid tissues (3). CXCR5 has also been shown to be highly expressed in primary breast tumors, in correlation with their propensity to grow and metastasize (4).
Limited Uses
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