CXXC1 (D1R5R) Rabbit Monoclonal Antibody #40672
- WB
- IP
- ChIP
- C&R
- C&T
Product Specifications
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 82 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
- C&T-CUT & Tag
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:100 |
| CUT&RUN | 1:100 |
| CUT&Tag | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
- Xu, C. et al. (2011) Nat Commun 2, 227.
- Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
- Miller, T. et al. (2001) Proc Natl Acad Sci U S A 98, 12902-7.
- Clouaire, T. et al. (2012) Genes Dev 26, 1714-28.
- Thomson, J.P. et al. (2010) Nature 464, 1082-6.
- Carlone, D.L. et al. (2005) Mol Cell Biol 25, 4881-91.
- Tate, C.M. et al. (2009) Mol Cell Biol 29, 3817-31.
- Young, S.R. et al. (2006) J Biol Chem 281, 37034-44.
- Butler, J.S. et al. (2008) DNA Cell Biol 27, 533-43.
Alternate Names
2410002I16Rik; 5830420C16Rik; CFP1; CGBP; CpG binding protein; CpG-binding protein; CXXC finger 1 (PHD domain); CXXC finger protein 1; CXXC-type zinc finger protein 1; CXXC1; DNA-binding protein with PHD finger and CXXC domain; hCGBP; HsT2645; PCCX1; PHD finger and CXXC domain-containing protein 1; PHF18; SPP1; ZCGPC1; zinc finger, CpG binding-type containing 1
Limited Uses
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