Fresh savings just popped up! 25% off 3 or more products. | Start Saving >>
9869
Cyclin Antibody Sampler Kit

Cyclin Antibody Sampler Kit #9869

 Image 1

Western blot analysis of extracts from various cell lines using Cyclin D2 (D52F9) Rabbit mAb #3741.

Learn more about how we get our images
Western Blotting Image 2

Western analysis of extracts from Hela cells that were untreated, treated with doxorubicin (0.5 µM, 24 hours), or with nocodazole (50 ng/ml, 24 hours), using Cyclin A2 (BF683) Mouse mAb.

Learn more about how we get our images
Western Blotting Image 3

Western blot analysis of extracts from various cell types using Cyclin B1 Antibody.

Learn more about how we get our images
Western Blotting Image 4

Western blot analysis of extracts from MCF7, L929 and C6 cells, using Cyclin D1 (92G2) Rabbit mAb.

Learn more about how we get our images
Western Blotting Image 5

Western blot analysis of extracts from various cell lines using Cyclin D2 (D52F9) Rabbit mAb.

Learn more about how we get our images
Western Blotting Image 6

Western blot analysis of extracts from SK-N-MC, C6 and IMCD3 cells, using Cyclin D3 (DCS22) Mouse mAb.

Learn more about how we get our images
Western Blotting Image 7

Western blot analysis of extracts from various cell types using Cyclin E (HE12) Mouse mAb.

Learn more about how we get our images
Western Blotting Image 8

Western blot analysis of extracts from MCF-7, SK-N-MC and HeLa cells, untreated or treated with the proteasome inhibitor MG-132, using Cyclin E2 Antibody.

Learn more about how we get our images
Western Blotting Image 9

Western blot analysis of extracts from Jurkat and Raji cells, using Cyclin H Antibody.

Learn more about how we get our images
Western Blotting Image 10

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Learn more about how we get our images
IF-IC Image 11

Confocal immunofluorescent analysis of HeLa cells using Cyclin B1 Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red) showing concentrated cyclin B1 staining surrounding mitotic chromatin.

Learn more about how we get our images
IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Cyclin D1 (92G2) Rabbit mAb.

Learn more about how we get our images
IHC-P (paraffin) Image 13

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cyclin D3 (DCS22) Mouse mAb.

Learn more about how we get our images
IHC-P (paraffin) Image 14

Immunohistochemical analysis of paraffin-embedded Apc (min/+) mouse intestine using Cyclin D1 (92G2) Rabbit mAb.

Learn more about how we get our images
IHC-P (paraffin) Image 15

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Cyclin D3 (DCS22) Mouse mAb.

Learn more about how we get our images
IHC-P (paraffin) Image 16

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cyclin D1 (92G2) Rabbit mAb in the presence of control peptide (left) or Cyclin D1 Blocking Peptide #1044 (right).

Learn more about how we get our images
IHC-P (paraffin) Image 17

Immunohistochemical analysis of paraffin-embedded H1975 xenograft, using Cyclin D1 (92G2) Rabbit mAb.

Learn more about how we get our images
Flow Cytometry Image 18

Flow cytometric analysis of untreated HT29 cells, using Cyclin D1 (92G2) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Learn more about how we get our images
Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Cyclin A2 (BF683) Mouse mAb 4656 20 µl
  • WB
H 55 Mouse IgE
Cyclin B1 Antibody 4138 20 µl
  • WB
  • IF
H M R Hm Mk 55 Rabbit 
Cyclin D1 (92G2) Rabbit mAb 2978 20 µl
  • WB
  • IHC
  • F
H M R 36 Rabbit IgG
Cyclin D2 (D52F9) Rabbit mAb 3741 20 µl
  • WB
  • IP
H M R 31 Rabbit IgG
Cyclin D3 (DCS22) Mouse mAb 2936 20 µl
  • WB
  • IHC
H M R 31 Mouse IgG1
Cyclin E1 (HE12) Mouse mAb 4129 20 µl
  • WB
H Mk 48 Mouse IgG1
Cyclin E2 Antibody 4132 20 µl
  • WB
H 48 Rabbit 
Cyclin H Antibody 2927 20 µl
  • WB
  • IP
H M R 36 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

The Cyclin Antibody Sampler Kit provides an economical means of evaluating the presence of cyclin proteins in cells. The kit contains enough primary and secondary antibodies to perform two western blot experiments with each antibody.

Each antibody in the Cyclin Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other cyclin proteins.

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues within the carboxy terminus of cyclin D1, the amino terminus of cyclin D2, recombinant human cyclin E1, or cyclin A2. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues within the carboxy terminus of cyclin H, cyclin E2, or the amino terminus of cyclin B. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Control of the cell cycle is regulated by a multitude of cellular events and processes. The cyclin dependent kinases (CDK) regulate many of these pathways and constitute an active complex when associated with their cyclin partners. This activity is controlled primarily by phosphorylation, which determines subcellular localization of the CDK/cyclin complex (1,2). Some phosphorylation events control the function of cytoplasmic retention sequences while other events regulate nuclear localization and export sequence function (3,4). Cyclin and cyclin-dependent kinase inhibitor (CKI) levels are regulated by ubiquitination and degradation via the ubiquitin proteasome pathway (5). A variety of CKI proteins associate with these complexes and modulate access to regulatory domains on cyclins (6). Additional complexity is generated as the controlled protein levels of each cyclin oscillate with the stages of cell cycle. Increased expression of Cyclin D1 is associated with certain types of cancer (7,8) and may associate with TSC2 (tuberin) independent of its Cdk partner (9).

  1. Krempler, A. et al. (2005) Cell Mol Life Sci 62, 1379-87.
  2. Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.
  3. Toyoshima, F. et al. (1998) EMBO J 17, 2728-35.
  4. Sherr, C.J. (1996) Science 274, 1672-7.
  5. Porter, L.A. and Donoghue, D.J. (2003) Prog Cell Cycle Res 5, 335-47.
  6. Benzeno, S. et al. (2006) Oncogene 25, 6291-303.
  7. Diehl, J.A. et al. (1997) Genes Dev 11, 957-72.
  8. Kim, J.K. and Diehl, J.A. (2009) J Cell Physiol 220, 292-6.
  9. Zacharek, S.J. et al. (2005) Cancer Res 65, 11354-60.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.

Subscribe