Cyclin E1 Antibody

Inquiry Info. # 2925

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Product Specifications

REACTIVITY
SENSITIVITY
MW (kDa)	51
SOURCE	Rabbit

Product Information

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Cyclin E1 Antibody detects endogenous levels of total cyclin E1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the amino terminus of human cyclin E1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Cyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. When conditions are favorable for cell cycle progression, cyclin D/CDK4/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E/CDK2 (1,2). Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380, and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7).

Alternate Names

CCNE; CCNE1; cyclin E1; cyclin Es; cyclin Et; G1/S-specific cyclin-E1; pCCNE1

Database Links

UniProt ID: P24864

Entrez-Gene Id: 898

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.

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Cyclin E1 Antibody #2925