Cell Signaling Technology Logo - Extra Large

CYP3A4 Antibody #12249

Filter:
  • WB
  • IP

Inquiry Info. # 12249

Please see our recommended alternatives.

    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 50
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CYP3A4 Antibody recognizes endogenous levels of total CYP3A4 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CYP3A4 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Cytochrome P450, subfamily 3A, polypeptide 4 (CYP3A4) is a mono-oxygenase enzyme (1) found in the endoplasmic reticulum membrane of liver and prostate microsomes. It is an important enzyme in drug metabolism; it catalyzes phase I oxidation reactions in approximately 50% of drugs in the modern market, as well as steroids, fatty acids, and some carcinogens (2). It is also involved in steroid and cholesterol synthesis (3,4). Expression of CYP3A4 can be induced by glucocorticoids, carcinogens, pesticides, and drugs, which can lead to drug interactions and toxicity (5).

    Alternate Names

    1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase; Albendazole monooxygenase (sulfoxide-forming); Albendazole sulfoxidase; Cholesterol 25-hydroxylase; CP33; CP34; CP3A4; CYP3A; CYP3A3; CYP3A4; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 3A4; cytochrome P450 family 3 subfamily A member 4; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; cytochrome P450, family 3, subfamily A, polypeptide 4; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; glucocorticoid-inducible P450; HLP; MGC126680; NF-25; Nifedipine oxidase; P450-III, steroid inducible; P450C3; P450PCN1; Quinine 3-monooxygenase; Taurochenodeoxycholate 6-alpha-hydroxylase

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.