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CYP51A1 (F5M4N) Rabbit Monoclonal Antibody #31966

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  • WB

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 57
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CYP51A1 (F5M4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total CYP51A1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu417 of human CYP51A1 protein.

    Background

    Cytochrome P450 family 51 subfamily A member 1 (CYP51A1), also known as lanosterol 14-alpha demethylase (LDM), is a key endoplasmic reticulum enzyme in cholesterol biosynthesis. Decreased activity of this crucial enzyme impairs liver function, leading to metabolism-associated liver disease and hepatocellular carcinoma (1). Furthermore, studies show that this enzyme, along with several other components in distal cholesterol biosynthesis, is a potential suppressor of ferropotosis (2). Additionally, CYP51A1 was shown to suppress alkalization-induced cell death in a lysosomal pathway in pancreatic cancer cells, demonstrating this enzyme to be a vulnerability in those cells (3). CYP51A1 was also identified as a plausible candidate gene for cerebral palsy (CP) (4).

    Alternate Names

    CP51; CP51A; CYP51; CYP51A1; CYPL1; CYPLI; Cytochrome P450 51A1; cytochrome P450 family 51 subfamily A member 1; Cytochrome P450-14DM; cytochrome P450, 51 (lanosterol 14-alpha-demethylase); cytochrome P450, family 51, subfamily A, polypeptide 1; Cytochrome P45014DM; Cytochrome P450LI; Lanosterol 14-alpha demethylase; lanosterol 14-alpha-demethylase; LDM; P450-14DM; P450L1; Sterol 14-alpha demethylase

    For Research Use Only. Not for Use in Diagnostic Procedures.
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