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8614
Cytoskeletal Marker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Cytoskeletal Marker Antibody Sampler Kit #8614

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Cytoskeletal Marker Antibody Sampler Kit: Image 1
Western blot analysis of extracts from COS-7, NIH/3T3 and PC12 cells, using β-Tubulin (9F3) Rabbit mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 2
Western blot analysis of extracts from A-431, HeLa, and A549 cells using Keratin 17 (D73C7) Rabbit mAb. As expected, A549 cells are negative for keratin 17.
Cytoskeletal Marker Antibody Sampler Kit: Image 3
Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 4
Western blot analysis of extracts from C2C12 cells, rat heart and human heart using Desmin (D93F5) XP® Rabbit mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 5
Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP® Rabbit mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 6
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Cytoskeletal Marker Antibody Sampler Kit: Image 7
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.
Cytoskeletal Marker Antibody Sampler Kit: Image 8
Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 9
Immunohistochemical analysis of paraffin-embedded human glioblastoma using β-Tubulin (9F3) Rabbit mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 10
Confocal immunofluorescent analysis of HeLa (left) and A549 (right) cells using Keratin 17 (D73C7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Cytoskeletal Marker Antibody Sampler Kit: Image 11
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 12
Confocal immunofluorescent analysis of mouse skeletal muscle using Desmin (D93F5) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Cytoskeletal Marker Antibody Sampler Kit: Image 13
Western blot analysis of extracts from control HeLa cells (lane 1) or Vimentin knockout HeLa cells (lane 2) using Vimentin (D21H3) XP® Rabbit mAb #5741 (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the Vimentin knockout HeLa cells confirms specificity of the antibody for Vimentin.
Cytoskeletal Marker Antibody Sampler Kit: Image 14
Confocal immunofluorescent analysis of HeLa cells using β-Actin (D6A8) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Cytoskeletal Marker Antibody Sampler Kit: Image 15
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Tubulin (9F3) Rabbit mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 16
Flow cytometric analysis of A549 cells (red) and HeLa cells (blue) using Keratin 17 (D73C7) Rabbit mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 17
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 18
Confocal immunofluorescent analysis of choroid plexus in mouse brain using Desmin (D93F5) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Ras (E4K9L) Rabbit mAb (Alexa Fluor® 647 Conjugate) #37182 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Cytoskeletal Marker Antibody Sampler Kit: Image 19
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Vimentin (D21H3) XP® Rabbit mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 20
Flow cytometric analysis of HeLa cells using β-Actin (D6A8) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP®Isotype control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Cytoskeletal Marker Antibody Sampler Kit: Image 21
Immunohistochemical analysis of paraffin-embedded human melanoma using β-Tubulin (9F3) Rabbit mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 22
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 23
Confocal immunofluorescent analysis of mouse liver at low (left) or high magnification (right) using Desmin (D93F5) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Ras (E4K9L) Rabbit mAb (Alexa Fluor® 647 Conjugate) #37182 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Cytoskeletal Marker Antibody Sampler Kit: Image 24
Immunohistochemical analysis of paraffin-embedded human tonsil using Vimentin (D21H3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Cytoskeletal Marker Antibody Sampler Kit: Image 25
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb preincubated with control peptide (left) or β-Tubulin Blocking Peptide #1032 (right).
Cytoskeletal Marker Antibody Sampler Kit: Image 26
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 27
Confocal immunofluorescent analysis of mouse pancreas using Desmin (D93F5) XP® Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using Ras (E4K9L) Rabbit mAb (Alexa Fluor® 647 Conjugate) #37182 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Cytoskeletal Marker Antibody Sampler Kit: Image 28
Immunohistochemical analysis of paraffin-embedded mouse colon using Vimentin (D21H3) XP® Rabbit mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 29
Confocal immunofluorescent analysis of HeLa cells using β-Tubulin (9F3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Cytoskeletal Marker Antibody Sampler Kit: Image 30
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 31
Confocal immunofluorescent analysis of SNB19 cells using Vimentin (D21H3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Cytoskeletal Marker Antibody Sampler Kit: Image 32
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Pan-Keratin (C11) mouse mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 33
Confocal immunofluorescent analysis of C2C12 cells (left, positive) or Neuro-2a cells (right, negative), using Desmin (D93F5) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Cytoskeletal Marker Antibody Sampler Kit: Image 34
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Pan-Keratin (C11) mouse mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 35
Flow cytometric analysis of MCF7 cells (blue) and HeLa cells (green) using Vimentin (D21H3) XP® Rabbit mAb(solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Cytoskeletal Marker Antibody Sampler Kit: Image 36
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Pan-Keratin (C11) mouse mAb.
Cytoskeletal Marker Antibody Sampler Kit: Image 37
Flow cytometric analysis of HeLa cells, using Vimentin (D21H3) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).
Cytoskeletal Marker Antibody Sampler Kit: Image 38
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Cytoskeletal Marker Antibody Sampler Kit: Image 39
Flow cytometric analysis of A-431 cells using Pan-Keratin (C11) Mouse mAb (solid line) versus a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed line). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.
To Purchase # 8614
Cat. # Size Qty. Price
8614T
1 Kit  (6 x 20 microliters) $ 458

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Desmin (D93F5) XP® Rabbit mAb 5332 20 µl
  • WB
  • IF
H M R 53 Rabbit IgG
Keratin 17 (D73C7) Rabbit mAb 4543 20 µl
  • WB
  • IF
  • F
H M R 48 Rabbit IgG
Pan-Keratin (C11) Mouse mAb 4545 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 46-58 Mouse IgG1
β-Tubulin (9F3) Rabbit mAb 2128 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk Z B 55 Rabbit IgG
Vimentin (D21H3) XP® Rabbit mAb 5741 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 57 Rabbit IgG
β-Actin (D6A8) Rabbit mAb 8457 20 µl
  • WB
  • IF
  • F
H M R Mk Dm Z 45 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

Product Description

The Cytoskeletal Marker Antibody Sampler Kit provides an economical means to evaluate the presence and status of select cytoskeleton associated proteins. The kit provides enough primary antibodies to perform two western blots per primary antibody.
MW (kDa) N/A

Specificity / Sensitivity

Desmin (D93F5) XP® Rabbit mAb, Keratin 17 (D73C7) Rabbit mAb, β-Tubulin (9F3) Rabbit mAb, and Vimentin (D21H3) XP® Rabbit mAb, and B-Actin (D68) Rabbit mAb recognize endogenous levels of total respective target proteins. Pan-Keratin (C11) Mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13, and 18. This antibody does not cross-react with other keratins.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide cooresponding to residues near the amino terminus of human β-actin protein, carboxy terminal residues of human desmin protein, residues near the carboxy terminus of human keratin 17 protein, the amino terminus of human β-tubulin protein, or residues surrounding Arg45 of human vimentin protein. Mouse monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A-431 cells.

Background

The cytoskeleton consists of three different types of cystosolic fibers: microtubules, microfilaments (actin) and intermediate filaments. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Major types of intermediate filaments are distinguished in part by the tissue in which they are expressed, for example; cytokeratins (epithelial cells), vimentin (mesenchyme origin), and desmin (skeletal, visceral and certain vascular smooth muscle cells) (2). Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form intermediate filaments (3). Research studies have demonstrated that vimentin is present in sarcomas, but not carcinomas, and its expression is examined relative to other markers in order to distinguish between the two forms of neoplasm (4). Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs (5). α/β-tubulin heterodimers form the tubulin subunit that comprises the microtubule building block (6).


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  1. Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.
  2. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
  3. Moll, R. et al. (1982) Cell 31, 11-24.
  4. Leader, M. et al. (1987) Histopathology 11, 63-72.
  5. Li, Z. et al. (1996) Dev Biol 175, 362-6.
  6. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

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For Research Use Only. Not For Use In Diagnostic Procedures.
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