DC-SIGN (D7F5C) XP® Rabbit mAb #13193
- WB
- IP
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45-55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 |
Storage
For a carrier free (BSA and azide free) version of this product see product #98584.
Protocol
Specificity / Sensitivity
DC-SIGN (D7F5C) XP® Rabbit mAb recognizes endogenous levels of total DC-SIGN protein. This antibody does not cross-react with DC-SIGNR.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DC-SIGN protein.
Background
DC-SIGN (CD209, CLEC4L) is a C-type lectin receptor expressed by dendritic cells (DCs) (1,2). The DC-SIGN transcript can undergo several splicing events to generate at least thirteen different transmembrane and soluble isoforms (3). DC-SIGN responds to a broad range of pathogens due to its ability to recognize both mannose and fructose carbohydrates, and is well studied for its role in HIV infection. Recognition of the HIV envelope glycoprotein gp120 by DC-SIGN leads to internalization of HIV by DCs and facilitates transmission of the virus to CD4+ T cells (2,4). DC-SIGN also mediates adhesion to T cells through interaction with ICAM-3, as well as transmigration across the endothelium by binding to ICAM-2 (1,5). The DC-SIGN receptor can modulate TLR signaling by activating the kinase Raf-1 (6,7). The closely related molecule DC-SIGNR (L-SIGN, CLEC4M) is 77% homologous to DC-SIGN and likely arose through a gene duplication event (8). Like DC-SIGN, DC-SIGNR binds mannose carbohydrates on the surface of pathogens (8,9). However, the expression patterns of the two receptors differ, as DC-SIGNR expression is restricted to endothelial cells of the liver, lymph node, and placenta (10). Murine cells contain a set of related molecules, SIGNR1-SIGNR8 (11). Based on sequence analysis, there is no clear murine ortholog to human DC-SIGN, however SIGNR3 is the most functionally similar due to its ability to recognize both mannose and fructose structures (11).
- Geijtenbeek, T.B. et al. (2000) Cell 100, 575-85.
- Geijtenbeek, T.B. et al. (2000) Cell 100, 587-97.
- Mummidi, S. et al. (2001) J Biol Chem 276, 33196-212.
- Kwon, D.S. et al. (2002) Immunity 16, 135-44.
- Geijtenbeek, T.B. et al. (2000) Nat Immunol 1, 353-7.
- Gringhuis, S.I. et al. (2007) Immunity 26, 605-16.
- Gringhuis, S.I. et al. (2010) Nat Immunol 11, 419-26.
- Bashirova, A.A. et al. (2001) J Exp Med 193, 671-8.
- Mitchell, D.A. et al. (2001) J Biol Chem 276, 28939-45.
- Pöhlmann, S. et al. (2001) Proc Natl Acad Sci U S A 98, 2670-5.
- Powlesland, A.S. et al. (2006) J Biol Chem 281, 20440-9.
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