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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. DDX39A (F7T4J) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DDX39A (F7T4J) Rabbit mAb #47857

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 49
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DDX39A (F7T4J) Rabbit mAb recognizes endogenous levels of total DDX39A protein. This antibody does not cross-react with UAP56 protein.


    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro22 of human DDX39A protein.

    Background

    The UAP56 gene is found in the central MHC region and encodes a member of the DEAD-box family of RNA helicases (1). Also known as DDX39B and BAT1, UAP56 functions as an ATP-dependent splicing factor and RNA helicase in the evolutionary conserved transcription/export (TREX) complex. The TREX complex is recruited to sites of active transcription, where it travels along the length of the gene with RNA polymerase II and exports resulting mRNAs to the cytoplasm (2-8).

    DDX39, also known as DDX39A, is a DEAD-box helicase highly homologous to UAP56 and is upregulated in lung squamous cell cancers (9,10). DDX39 has been shown to bind to TRF2 to regulate telomere protection (11). Furthermore, DDX39 is overexpressed in several cancer types, which is associated with poor prognosis (12-15). Both UAP56 and DDX39 are hijacked by various viral replication machineries to enable viral reproduction and mRNA export (16-18). UAP56 and DDX39 have also been implicated in promoting the AR-V7 splice variant in advanced prostate cancers (19).

    1. Peelman, L.J. et al. (1995) Genomics 26, 210-8.
    2. Fleckner, J. et al. (1997) Genes Dev 11, 1864-72.
    3. Strässer, K. et al. (2002) Nature 417, 304-8.
    4. Custódio, N. et al. (2004) RNA 10, 622-33.
    5. Kapadia, F. et al. (2006) Gene 384, 37-44.
    6. Shen, J. et al. (2007) J Biol Chem 282, 22544-50.
    7. Kota, K.P. et al. (2008) J Cell Sci 121, 1526-37.
    8. Majerciak, V. et al. (2010) Virology 407, 206-12.
    9. Sugiura, T. et al. (2007) Exp Cell Res 313, 782-90.
    10. Sugiura, T. et al. (2007) Cancer Biol Ther 6, 957-64.
    11. Yoo, H.H. and Chung, I.K. (2011) Aging Cell 10, 557-71.
    12. Kikuta, K. et al. (2012) J Proteomics 75, 1089-98.
    13. Kuramitsu, Y. et al. (2013) Anticancer Res 33, 2557-60.
    14. Kuramitsu, Y. et al. (2013) Anticancer Res 33, 3133-6.
    15. Xing, C. et al. (2020) Front Oncol 10, 1261.
    16. Dufu, K. et al. (2010) Genes Dev 24, 2043-53.
    17. Zielke, B. et al. (2011) J Virol 85, 1804-19.
    18. Wisskirchen, C. et al. (2011) J Virol 85, 8646-55.
    19. Nakata, D. et al. (2017) Biochem Biophys Res Commun 483, 271-276.

    Database Links

    UniProt ID: O00148


    Entrez-Gene Id: 10212


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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