DDX5 (D15E10) XP® Rabbit mAb (BSA and Azide Free) #75466
- WB
- IF
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 68 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
DDX5 (D15E10) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total DDX5 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Bovine, Horse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser498 of human DDX5 protein.
Background
DDX5 (DEAD box polypeptide 5), also known as p68, was first identified as a 68 kDa nuclear protein with similarity to translation initiation factor eIF-4A (1). DDX5 is a member of the DEAD box family of putative RNA helicases, defined by the presence of a conserved DEAD (Asp-Glu-Ala-Asp) motif that appears to function primarily in the regulation of RNA secondary structure. DDX5 exhibits ATP-dependent RNA helicase activity (2) and has been identified as a critical subunit of the DROSHA complex that regulates miRNA and rRNA processing (3,4). DDX may also regulate mRNA splicing (5) and has been shown to interact with HDAC1, where it can regulate promoter-specific transcription (6). DDX5 interacts with a diverse group of proteins, including Runx2, p53, Smad3, CBP, and p300 (7-10), suggesting an important role for DDX5 in a multitude of developmental processes. Notably, DDX5 may be involved in growth factor-induced epithelial mesechymal transition (EMT). Phosphorylation of DDX5 at Tyr593 following PDGF stimulation was shown to displace Axin from β-catenin; this prevented phosphorylation of β-catenin by GSK-3β, leading to Wnt-independent nuclear translocation of β-catenin (11) and increased transcription of c-Myc, cyclin D1, and Snai1 (12,13).
- Ford, M.J. et al. (1988) Nature 332, 736-8.
- Hirling, H. et al. (1989) Nature 339, 562-4.
- Fukuda, T. et al. (2007) Nat Cell Biol 9, 604-11.
- Davis, B.N. et al. (2008) Nature 454, 56-61.
- Camats, M. et al. (2008) PLoS ONE 3, e2926.
- Wilson, B.J. et al. (2004) BMC Mol Biol 5, 11.
- Jensen, E.D. et al. (2008) J Cell Biochem 103, 1438-51.
- Bates, G.J. et al. (2005) EMBO J 24, 543-53.
- Warner, D.R. et al. (2004) Biochem Biophys Res Commun 324, 70-6.
- Rossow, K.L. and Janknecht, R. (2003) Oncogene 22, 151-6.
- Yang, L. et al. (2006) Cell 127, 139-55.
- Yang, L. et al. (2007) J Biol Chem 282, 16811-9.
- Carter, C.L. et al. (2010) Oncogene 29, 5427-36.
Limited Uses
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