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Deltex-2 Antibody #2663

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  • IP

Inquiry Info. # 2663

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    Product Specifications

    REACTIVITY M
    SENSITIVITY Transfected Only
    MW (kDa) 70
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Deltex-2 Antibody detects transfected levels of total Deltex-2 protein.

    Species Reactivity:

    Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Human, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala607 of human Deltex-2. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Deltex (DTX) encodes a putative E3 ubiquitin ligase first identified from Drosophila and shown to modulate Notch signaling (1). Several mammalian Deltex homologs have been isolated (2,3), all containing a pair of WWE repeats, a proline-rich region, and a Ring-finger domain (4,5). Deltex binds to the ankyrin-repeat domain of Notch and is capable of regulating Notch signaling positively or negatively in different systems (4,6). In addition, Deltex can also form homo-multimers (4). While Deltex has been implicated in Notch signaling regulation of lymphocyte development, this signal modifier may also have a transcriptional function independent of Notch (3, 6-8).

    Alternate Names

    deltex 2, E3 ubiquitin ligase; deltex E3 ubiquitin ligase 2; deltex homolog 2; deltex homolog 2 (Drosophila); Deltex2; DTX2; hDTX2; KIAA1528; MGC71098; Probable E3 ubiquitin-protein ligase DTX2; Protein deltex-2; RING finger protein 58; RING-type E3 ubiquitin transferase DTX2; RNF58; zinc ion binding protein

    For Research Use Only. Not for Use in Diagnostic Procedures.
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