Render Target: STATIC
Render Timestamp: 2024-10-04T10:05:26.471Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DFCP1 (E9R6P) Rabbit mAb #85156

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 82
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DFCP1 (E9R6P) Rabbit mAb recognizes endogenous levels of total DFCP1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val451 of human DFCP1 protein.

    Background

    Double FYVE-containing protein 1 (DFCP1; gene name ZFYVE1), which was identified from a human bone marrow library, contains two carboxyl terminal FYVE domains that function as binding sites for phosphatidylinositol 3-phosphate (PI3P) (1). PI3P generated predominantly by the class III PI3-kinase VPS34 plays a key role in membrane trafficking as well as autophagy (2,3). DFCP1 is primarily localized to the Golgi and endoplasmic reticulum (ER) (4,5). However, during autophagy DFCP1 re-localizes to subdomains of the ER, the omegasome, which become the sites for autophagosome formation (6,7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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