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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DGKζ (F6W7N) Rabbit mAb #80182

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 110, 140
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DGKζ (F6W7N) Rabbit mAb recognizes endogenous levels of total DGKζ protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DGKζ protein.

    Background

    Diacylglycerol kinases (DGKs) are a unique family of enzymes that phosphorylate the signaling molecule diacylglycerol (DAG) and convert it to phosphatidic acid (PA) (1). DGKs affect various cellular processes by regulating DAG and PA concentrations, including immune signaling, glucose uptake, and neuronal remodeling (2). There are ten DGK isoforms in mammals (α, β, γ, δ, ε, ζ, η, θ, ι, and κ); these isoforms share a catalytic domain but differ in subcellular localization, tissue specificity, and overall function (3). DGK isoform zeta (DGKζ) is one of the primary isoforms expressed in T cells, where it negatively regulates TCR signaling (4). DGKζ attenuates TCR activation by blocking DAG-mediated signaling of downstream effector molecules, which can result in T cell anergy (5,6). Knockout or inhibition of DGKζ has been shown to reverse T cell anergy and enhance the cytotoxicity of chimeric antigen receptor T cells (CAR-Ts), suggesting that DGKζ can be targeted for immunotherapies (7,8). DGKζ may also play a role in cancer, where it is associated with cell proliferation and metastasis in cervical and triple-negative breast cancer (9,10).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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