Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DHCR7 (F7I5B) Rabbit Monoclonal Antibody #62115

Filter:
  • WB
  • IF

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 40
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DHCR7 (F7I5B) Rabbit mAb recognizes endogenous levels of total DHCR7 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DHCR7 protein.

    Background

    7-dehydrocholesterol reductase (DHCR7) catalyzes the conversion of 7-dehydrocholesterol (7-DHC) to cholesterol, the last step of cholesterol biosynthesis. Studies show that DHCR7 expression is downregulated in macrophages upon viral infection. DHCR7 deficiency activates the PI3K-AKT3 pathway. AKT3 phosphorylates interferon regulatory factor 3 (IRF3) at Ser385, leading to IRF3 activation and increased type I interferon (IFN-I) production in macrophages (1). In addition, DHCR7 has proferroptotic activity. Its substrate, 7-DHC, suppresses ferroptosis by inhibiting phospholipid peroxidation. Blocking 7-DHC biosynthesis enhances ferroptosis of cancer cells (2,3).

    Alternate Names

    7-dehydrocholesterol reductase; 7-DHC reductase; D7SR; delta-7-dehydrocholesterol reductase; Delta7-sterol reductase; DHCR7; Putative sterol reductase SR-2; SLOS; sterol delta-7-reductase; Sterol Delta(7)-reductase; Sterol reductase SR-2

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.