Render Target: STATIC
Render Timestamp: 2024-07-26T10:49:54.265Z
View Featured Offers >>
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. DHX9 (E6M4Q) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DHX9 (E6M4Q) Rabbit mAb #71286

Filter:
  • WB
  • ChIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 145
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Chromatin IP 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DHX9 (E6M4Q) Rabbit mAb recognizes endogenous levels of total DHX9 protein.


    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile23 of human DHX9 protein.

    Background

    DExH-box helicase 9 (DHX9), also known as nuclear DNA helicase II or RNA helicase A, is a protein involved in unwinding DNA and RNA by using NTP (1). DHX9 can translocate in the 3’ to 5’ direction and can utilize all dNTPs or rNTPs, and contributes to pre-mRNA splicing (2,3). Essential for embryo development, DHX9 plays roles in many biological processes, including DNA replication, self-renewal, dosage compensation, and neuronal development (4-7). DHX9 is a critical regulator of abnormal DNA secondary structures (8). It is recruited by TDRD3 to R-loops, where it can unwind and resolve them to prevent DNA damage (9-12). DHX9 plays a central role in the formation and resolution of R-loops (8-11). It also can bind to G-quadruplexes and inverted-repeat Alu elements, suppressing formation of circular RNAs (9,13). BRCA1 is recruited to RNA by DHX9 to promote homologous recombination (14). Furthermore, DHX9 has been shown to be upregulated in various cancer types and can also play a role in viral replication (15-17).

    1. Zhang, S. and Grosse, F. (1994) Biochemistry 33, 3906-12.
    2. Lee, C.G. and Hurwitz, J. (1992) J Biol Chem 267, 4398-407.
    3. Zhang, S. et al. (1999) J Cell Sci 112 (Pt 7), 1055-64.
    4. Friedemann, J. et al. (2005) J Biol Chem 280, 31303-13.
    5. Närvä, E. et al. (2012) Stem Cells 30, 452-60.
    6. Kuroda, M.I. et al. (1991) Cell 66, 935-47.
    7. Hoekstra, E.J. et al. (2013) PLoS One 8, e53122.
    8. Jain, A. et al. (2013) Nucleic Acids Res 41, 10345-57.
    9. Chakraborty, P. and Grosse, F. (2011) DNA Repair (Amst) 10, 654-65.
    10. Chakraborty, P. et al. (2018) Nat Commun 9, 4346.
    11. Cristini, A. et al. (2018) Cell Rep 23, 1891-1905.
    12. Yuan, W. et al. (2021) Nucleic Acids Res 49, 8573-8591.
    13. Aktaş, T. et al. (2017) Nature 544, 115-119.
    14. Chakraborty, P. and Hiom, K. (2021) Nat Commun 12, 4126.
    15. Shi, F. et al. (2021) J Clin Lab Anal 35, e24052.
    16. Xiao, L. et al. (2022) J Cancer 13, 102-111.
    17. Guo, F. and Xing, L. (2021) Virus Res 291, 198206.

    Database Links

    UniProt ID: Q08211


    Entrez-Gene Id: 1660


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.