Diap1 (E1E4K) Rabbit mAb #14634
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 150 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
mDia1/diap1 is activated by GTP-bound Rho, leading to Rho-associated kinase (ROCK)-dependent stress fiber formation (3,4). Rho activation of mDia1 has also been shown to regulate serum response factor (SRF)-dependent transcription (5), and has been implicated in human cancer phenotypes such as ras-mediated transformation, metastasis and invasion (reviewed in 6).
mDia3/diap2, activated by the Rho family small GTPase cdc42, regulates the attachment of microtubules to the kinetochore during mitosis in mammalian cells (7).
Rho-dependent activation of mDia2/diap3 is important in assembly of the contractile ring during cytokinesis (8,9).
- Schönichen, A. and Geyer, M. (2010) Biochim Biophys Acta 1803, 152-63.
- Chesarone, M.A. et al. (2010) Nat Rev Mol Cell Biol 11, 62-74.
- Watanabe, N. et al. (1999) Nat Cell Biol 1, 136-43.
- Ishizaki, T. et al. (2001) Nat Cell Biol 3, 8-14.
- Copeland, J.W. and Treisman, R. (2002) Mol Biol Cell 13, 4088-99.
- Narumiya, S. et al. (2009) Cancer Metastasis Rev 28, 65-76.
- Yasuda, S. et al. (2004) Nature 428, 767-71.
- Watanabe, S. et al. (2010) Mol Biol Cell 21, 3193-204.
- Watanabe, S. et al. (2008) Mol Biol Cell 19, 2328-38.
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