DLK1 (F7O3Q) Rabbit mAb (BSA and Azide Free) #47633
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
For standard formulation of this product see product #94845
Storage
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Humans and rodents express multiple DLK1 isoforms, which are either membrane-bound or contain an ADAM17/TACE cleavage site for release of the soluble ectodomain (8). As high DLK1 expression is pro-oncogenic in some contexts, differential isoform expression may promote cancer cell survival, with both the ectodomain and intracellular domain having distinct functions (9). Under hypoxic conditions, HIF proteins induce ADAM17/TACE cleavage and internalization of the DLK1 intracellular domain, which localizes to the nucleus and alters Akt and p53 signaling cascades in glioma (10). Hypoxia increases DLK1 expression, and phosphorylation of the DLK1 C-terminus at Tyr339 and Ser355 increases neuronal tumor sphere growth (11). Nuclear DLK1 directly interacts with tumor suppressor NCoR1, correlating with poor prognosis in non-small cell lung cancer (NSCLC) (12). DLK1 has emerged as a target for novel antibody drug conjugates (ADCs) in neuroblastoma and adrenocortical carcinoma (13,14).
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- Grassi, E.S. and Pietras, A. (2022) J Histochem Cytochem 70, 17-28.
- Pittaway, J.F.H. et al. (2021) Endocr Relat Cancer 28, R271-R287.
- Grassi, E.S. et al. (2020) Oncogene 39, 4028-4044.
- Kim, Y. et al. (2009) Cancer Res 69, 9271-80.
- Tan, J. et al. (2019) Biosci Rep 39, BSR20192362. doi: 10.1042/BSR20192362.
- Hamilton, A.K. et al. (2024) Cancer Cell 42, 1970-1982.e7.
- Sun, N.Y. et al. (2024) bioRxiv , 0.09.617077. doi: 10.1101/2024.10.09.617077.
Limited Uses
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