DMT1/SLC11A2 (D3V8G) Rabbit mAb #15083
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55, 70-100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
DMT1/SLC11A2 (D3V8G) Rabbit mAb recognizes endogenous levels of total DMT1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DMT1 protein.
Background
Divalent metal-ion transporter 1 (DMT1, SLC11A2, NRAMP2) is a transmembrane metal ion transport protein that plays critical roles in non-heme iron absorption in the intestine and iron acquisition by erythroid precursor cells (1,2). Following the cellular uptake of iron, DMT1 transfers ferric iron from the endosomes to the cytoplasm (3,4). The DMT1 protein can transport up to eight different metals, including iron, manganese, cobalt, and cadmium (5). Four mammalian DMT1 isoforms are expressed in various tissues and are differentially regulated at both the transcriptional and post-translational level (6,7). Mutations in the corresponding SLC11A2 gene can result in hypochromic microcytic anemia and iron overload. Aberrant iron transport in these individuals results in erythroid hyperplasia, high serum iron, and impaired liver function (8-10). Research studies show elevated DMT1 levels and iron accumulation in the substantia nigra of Parkinson's disease patients and the corresponding animal model (11,12).
- Gunshin, H. et al. (1997) Nature 388, 482-8.
- Canonne-Hergaux, F. et al. (2001) Blood 98, 3823-30.
- Canonne-Hergaux, F. et al. (1999) Blood 93, 4406-17.
- Hentze, M.W. et al. (2004) Cell 117, 285-97.
- Garrick, M.D. et al. (2003) Biometals 16, 41-54.
- Mackenzie, B. et al. (2007) Biochem J 403, 59-69.
- Garrick, M.D. et al. (2012) Biometals 25, 787-93.
- Mims, M.P. et al. (2005) Blood 105, 1337-42.
- Iolascon, A. et al. (2006) Blood 107, 349-54.
- Beaumont, C. et al. (2006) Blood 107, 4168-70.
- Salazar, J. et al. (2008) Proc Natl Acad Sci U S A 105, 18578-83.
- Howitt, J. et al. (2014) PLoS One 9, e87119.
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