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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-ATR (Ser428) Antibody 2853 20 µl
Western Blotting
H M R Mk 300 Rabbit 
Phospho-BRCA1 (Ser1524) Antibody 9009 20 µl
Western Blotting
H 220 Rabbit 
Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb 2197 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Flow Cytometry
H 62 Rabbit IgG
Phospho-Chk1 (Ser345) (133D3) Rabbit mAb 2348 20 µl
Western Blotting Immunofluorescence Flow Cytometry
H M R Mk 56 Rabbit IgG
Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb 9718 20 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 15 Rabbit IgG
Phospho-p53 (Ser15) (16G8) Mouse mAb 9286 20 µl
Western Blotting Immunofluorescence Flow Cytometry
H 53 Mouse IgG1
Phospho-ATM (Ser1981) (D6H9) Rabbit mAb 5883 20 µl
Western Blotting
H 350 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting

Product Description

This kit provides an economical means to analyze major signaling checkpoints in response to DNA damage. The kit contains primary and secondary antibodies to perform two Western blots with each antibody.

Specificity / Sensitivity

All antibodies in the DNA Damage Antibody Sampler Kit recognize their targets proteins only when modified at the indicated site.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 Kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). p53 is phosphorylated by ATM, ATR and DNA-PK at Ser15. This phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage (4,5). Chk1 and Chk2, downstream protein kinases of ATM/ATR, plays an important role in DNA damage checkpoint control, embryonic development and tumor suppression (6). Chk1 is phosphorylated at Ser280 and Ser296 following DNA damage. The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues, including Thr68, each followed by glutamine (SQ or TQ motif). After DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (7-9). The breast cancer susceptibility proteins BRCA1 and BRCA2 are frequently mutated in cases of hereditary breast and ovarian cancers and have roles in multiple processes related to DNA damage, repair, cell cycle progression, transcription, ubiquitination and apoptosis. Numerous DNA-damage induced phosphorylation sites on BRCA1 have been identified, including serine 1524, and kinases activated in a cell cycle-dependent manner, including Aurora A and CDK2, can also phosphorylate BRCA1. IR, DNA and radiometric-induced DNA damage also results in rapid phosphorylation of the histone H2A family member H2A.X at Ser139 by ATM (10,11). Within minutes following DNA damage, Ser139-phosphorylated H2A.X localizes to sites of DNA damage at subnuclear foci (12).

1.  Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.

2.  Abraham, R.T. DNA Repair (Amst) 3, 883-887.

3.  Rogakou, E.P. et al. (1998) J Biol Chem 273, 5858-68.

4.  Shechter, D. et al. DNA Repair (Amst) 3, 901-908.

5.  Burma, S. et al. (2001) J Biol Chem 276, 42462-7.

6.  Shieh, S.Y. et al. (1997) Cell 91, 325-34.

7.  Rogakou, E.P. et al. (1999) J Cell Biol 146, 905-16.

8.  Tibbetts, R.S. et al. (1999) Genes Dev 13, 152-7.

9.  Martinho, R.G. et al. (1998) EMBO J. 17, 7239-17249.

10.  Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389-10394.

11.  Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.

12.  Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

DNA Damage Antibody Sampler Kit