DNA Ligase III (F2A1H) Rabbit mAb #43640

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	100
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:10 - 1:50
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

DNA Ligase III (F2A1H) Rabbit mAb recognizes endogenous levels of total DNA ligase III protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human DNA ligase III protein.

Background

DNA ligase III/LIG3 is an ATP-dependent DNA ligase that functions in DNA repair. Four isoforms of LIG3 result from alternative splicing/initiation, including a mitochondrial-specific isoform essential for cell viability (1). Loss of nuclear LIG3 sensitizes resistant cells to PARP inhibitors and enhances PARP inhibitor efficacy in BRCA1-deficient mouse mammary tumors (2). In multiple myeloma (MM), LIG3 is crucial for repair of DNA double-stranded breaks (DSBs) via alternative-non-homologous end joining (Alt-NHEJ), and LIG3 inhibitors are being investigated as cancer therapeutics (3).

Database Links

UniProt ID: P49916

Entrez-Gene Id: 3980

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For Research Use Only. Not For Use In Diagnostic Procedures.

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