Interested in promotions? | Click here >>
66991
DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (BSA and Azide Free) #66991

Reviews ()
Citations (0)
Filter:
  1. WB
  2. IHC
Western Blotting Image 1: DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using DNAM-1/CD226 (E8L9G) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using DNAM-1/CD226 (E8L9G) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma (left) or ovarian serous carcinoma (right) using DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (top) or DNAM-1/CD226 Rabbit mAb (bottom). These two antibodies detect independent, unique epitopes on human DNAM-1/CD226. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human spleen using DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded 293T cell pellet, untransfected (left) or DNAM-1-transfected (right), using DNAM-1/CD226 (E8L9G) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded TALL-1 cell pellet (left, positive), HEL cell pellet (middle, positive), or H820 cell pellet (right, negative) using DNAM-1/CD226 (E8L9G) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 66991
Cat. # Size Qty. Price
66991SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 70
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #66631. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #66631

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total DNAM-1/CD226 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human DNAM-1/CD226 protein.

Background

DNAM-1/CD226 is a member of the immunoglobulin-like superfamily. It is expressed on T cells, natural killer (NK) cells, monocytes, and macrophages. It is a major activating receptor on NK cells. Engagement with its ligands Nectin-2 (CD112) and PVR (poliovirus receptor, also known as CD155) on the target cells leads to downstream activation of ERK, AKT, and PLCg2 signaling pathways that are crucial for NK activation and NK-mediated killing (1-4). LFA-1 physically associates with DNAM-1/CD226 and contributes to its signaling (5). Activating signal of DNAM-1/CD226 is counterbalanced by inhibitory receptors TIGIT and CD96, competing for the common ligands CD122 and PVR (6,7).
  1. Elishmereni, M. et al. (2008) Curr Opin Investig Drugs 9, 491-6.
  2. Xiong, P. et al. (2015) Immunology 146, 369-78.
  3. de Andrade, L.F. et al. (2014) Immunol Cell Biol 92, 237-44.
  4. Cifaldi, L. et al. (2019) Int J Mol Sci 20, pii: E3715. doi: 10.3390/ijms20153715.
  5. Shibuya, K. et al. (2003) J Exp Med 198, 1829-39.
  6. Stein, N. et al. (2017) Hum Antibodies 25, 111-9.
  7. Sanchez-Correa, B. et al. (2019) Cancers (Basel) 11, pii: E877. doi: 10.3390/cancers11060877.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.