Render Target: STATIC
Render Timestamp: 2024-10-11T09:44:55.488Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:58:54.906
Product last modified at: 2024-09-30T08:01:25.071Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. DOCK8 (E5Y9C) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DOCK8 (E5Y9C) Rabbit mAb #39263

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 220-240
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DOCK8 (E5Y9C) Rabbit mAb recognizes endogenous levels of total DOCK8 protein. This antibody recognizes DOCK8 isoforms 1-4.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DOCK8 protein.

    Background

    DOCK8 (dedicator of cytokinesis 8) is a member of the DOCK family of proteins that function as guanine nucleotide exchange factors (GEFs) for the Rho family of GTPases (1,2). DOCK8 is responsible for activation of the GTPase Cdc42, which exchanges bound GDP for free GTP, and plays a regulatory role in cell polarity and migration (2). DOCK8 immunodeficiency syndrome, or autosomal-recessive hyper-IgE syndrome, is caused by mutations of DOCK8 (3,4). Patients with this disorder have susceptibility to viral infections, eczema, increased serum IgE, defective T cell activation and differentiation, and impaired eosinophil homeostasis, due to T cell dysfunction associated with DOCK8 deficiency (2-4).

    Database Links

    UniProt ID: Q8NF50


    Entrez-Gene Id: 81704


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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