Render Target: STATIC
Render Timestamp: 2024-12-13T10:51:15.070Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-12-06 13:05:10.292
Product last modified at: 2024-12-09T16:30:09.525Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

DOG1/Anoctamin 1 (K9) Mouse mAb #54598

Filter:
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG2b
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    IHC Leica Bond 1:50 - 1:200
    Immunohistochemistry (Paraffin) 1:50 - 1:200

    Storage

    Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DOG1/Anoctamin 1 (K9) Mouse mAb recognizes endogenous levels of DOG1/Anoctamin 1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to the carboxy terminus of human DOG1/Anoctamin 1 protein.

    Background

    Anoctamin 1 is a plasma membrane calcium-activated chloride channel, also known as discovered on gastrointestinal stromal tumors protein 1 (DOG1) or transmembrane protein 16A (TMEM16A) (1). It is essential for chloride secretion from epithelial tissues (2) and has been shown to be a specific marker for cells of Cajal (3), the pacemaker cells that control smooth muscle contraction. Anoctamin 1 knockout mice exhibit an altered gastric smooth muscle rhythmic contraction (4). More recently, research studies have identified anoctamin 1 as a heat sensor in nociceptive neurons (5). Heat above 44ºC triggers anoctamin 1-dependent depolarization, contributing to the mediation of thermal nociception (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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