Render Target: STATIC
Render Timestamp: 2024-09-19T10:47:57.188Z
Commit: 195d6e018d1f29a59e5c111fed767382e8d7f01b
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DPF3/BAF45C (E7F7N) Rabbit mAb #82788

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H R
    SENSITIVITY Endogenous
    MW (kDa) 45, 47
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DPF3/BAF45C (E7F7N) Rabbit mAb recognizes endogenous levels of total DPF3/BAF45C protein. Based on sequence, this antibody should detect both DPF3a and DPF3b.

    Species Reactivity:

    Human, Rat

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu146 of human DPF3/BAF45C protein.

    Background

    The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM (1). This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant (1). SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle, and differentiation (1). Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors (2). Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression, including HDACs, Rb, and Tif1β (3-5). Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also plays a role as a tumor suppressor and Brg1 is mutated in several tumor cell lines (6-8). DPF3/BAF45C is a member of the SWI/SNF complex that binds to acetylated and methylated histone tails via its double PHD finger domains. There are two splice variants in human and mouse, DPF3a and DPF3b, which differ in their C-terminus as DPF3a contains a single truncated PHD finger. DPF3/BAF45C has been shown to be a key regulator in heart and muscle development, and its phosphorylation removes HEY transcriptional repressors to induce cardiac hypertrophy (9-11). DPF3 has also been implicated in brown adipogenesis and NF-κB signaling (12,13).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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