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dUTPase/DUT (F3X6Z) Rabbit mAb

dUTPase/DUT (F3X6Z) Rabbit mAb #70372

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Supporting Data

REACTIVITY	H M
SENSITIVITY	Endogenous
MW (kDa)	20
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

dUTPase/DUT (F3X6Z) Rabbit mAb recognizes endogenous levels of total dUTPase/DUT protein.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val188 of human dUTPase/DUT protein.

Background

dUTP pyrophosphatase (dUTPase), also known as deoxyuridine triphosphatase (DUT), catalyzes the conversion of dUTP to dUMP and pyrophosphate (1,2). Studies show that fumarate, an intermediate in the TCA/Krebs cycle, functions as a signaling metabolite to regulate mitochondrial biogenesis. Fumarate binds to the mitochondrial enzyme, malic enzyme 2 (ME2), and stimulates the formation of ME2 dimers. ME2 dimers, in turn, activate the enzymatic activity of dUTPase/DUT, promoting thymidine biosynthesis and increasing mitochondrial DNA replication (3).

Database Links

UniProt ID: P33316

Entrez-Gene Id: 1854

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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