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Dvl1 (F8K9L) Rabbit Monoclonal Antibody #17026

Filter:
  • WB
  • IP

    Product Specifications

    REACTIVITY H R
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Dvl1 (F8K9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total Dvl1 protein. This antibody detects a 140 kDa and a 180 kDa protein of unknown identity in some cell lines. The presence of these bands is observed in cell lysates predicted to be positive, but not in those predicted to be negative, suggesting that the bands are target-related.

    Species Reactivity:

    Human, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human Dvl1 protein.

    Background

    Dishevelled (Dsh) proteins are important intermediates of Wnt signaling pathways. Dsh inhibits glycogen synthase kinase-3β, promoting β-catenin stabilization. Dsh proteins also participate in the planar cell polarity pathway by acting through JNK (1,2). There are three Dsh homologs in mammals: Dvl1, Dvl2, and Dvl3. Upon treatment with Wnt proteins, Dvls become hyperphosphorylated (3) and accumulate in the nucleus (4). Dvl proteins also associate with actin fibers and cytoplasmic vesicular membranes (5) and mediate endocytosis of the Fzd receptor after Wnt protein stimulation (6). Overexpression of Dvl has been reported in certain cancers (7,8).

    Alternate Names

    dishevelled 1 (homologous to Drosophila dsh); dishevelled segment polarity protein 1; Dishevelled-1; dishevelled-1-like; dishevelled, dsh homolog 1; dishevelled, dsh homolog 1 (Drosophila); DRS2; DSH homolog 1; DSH homolog 1-like; DVL; DVL1; DVL1L1; DVL1P1; MGC54245; Segment polarity protein dishevelled homolog DVL-1; segment polarity protein dishevelled homolog DVL-1-like

    For Research Use Only. Not for Use in Diagnostic Procedures.
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