DYNLL1/LC8 (E6W7R) Rabbit mAb #82007
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 10 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
DYNLL1/LC8 (E6W7R) Rabbit mAb recognizes endogenous levels of total DYNLL1/LC8 protein. This antibody cross-reacts with DYNLL2.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala28 of human DYNLL1/LC8 protein.
Background
Hub proteins are important components of protein interaction networks, binding to a wide variety of proteins, and often driving dimerization (1). The dynein light chain LC8 family members, DYNLL1 and DYNLL2, are conserved, ubiquitously expressed hub proteins that interact with a diverse group of proteins, including molecular motor proteins. LC8 family member protein interactions regulate multiple cellular processes (2).
Loss of DYNLL1 in BRCA1-mutant cancer cells restores homologous recombination by allowing DNA end resection, conferring resistance to platinum and PARP inhibitor therapies (3). DYNLL1 regulation of DNA repair pathways likely occurs through its interaction with end resection machinery and 53BP1 (3,4). Upregulation of DYNLL1 has been associated with chemoresistance and progression of human cancer (5-7).
- Jespersen, N. and Barbar, E. (2020) Trends Biochem Sci 45, 375-384.
- Rapali, P. et al. (2011) FEBS J 278, 2980-96.
- He, Y.J. et al. (2018) Nature 563, 522-526.
- Becker, J.R. et al. (2018) Nat Commun 9, 5406.
- Berkel, C. and Cacan, E. (2021) Biochem Biophys Res Commun 570, 74-81.
- Berkel, C. and Cacan, E. (2020) Exp Mol Pathol 117, 104567.
- Wong, D.M. et al. (2016) Cell Rep 14, 1488-1499.
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