Dystrophin (E4F3S) Rabbit mAb #47759
- WB
- IHC
Supporting Data
| REACTIVITY | H R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 427 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
Dystrophin (E4F3S) Rabbit mAb recognizes endogenous levels of total dystrophin protein.
Species Reactivity:
Human, Rat
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human dystrophin protein.
Background
Dystrophin is a large, multi-domain protein that plays a critical role in muscle function that anchors the extracellular matrix to the cytoskeleton via F-actin (1). The protein is encoded in the DMD (Duchenne muscular dystrophy) gene located on the X chromosome. Dystrophin is part of a larger complex called the dystrophin-associated glycoprotein complex (DGC), which accumulates at the neuromuscular junction and synapses (2). The DGC also plays a structural function in muscle fibers by stabilizing the sarcolemma and protecting muscle cell membranes during muscle contractions (3). Mutations in the DMD gene, which is the largest known gene in humans, are genetically linked to Duchenne muscular dystrophy and Becker muscular dystrophy. Dystrophin may play a role in the proper function of central nervous system synapses, as neurological disorders are observed in Duchenne muscular dystrophy patients (4). Moreover, mutations in the DMD gene are linked to Alzheimer’s Disease, suggesting a role in neurodegeneration (5).
Limited Uses
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