E4BP4/NFIL3 (D5K8O) Rabbit mAb #14312
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
E4BP4/NFIL3 (D5K8O) Rabbit mAb recognizes endogenous levels of total E4BP4/NFIL3 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro455 of human E4BP4/NFIL3 protein.
Background
E4BP4/NFIL3 is a basic leucine zipper transcriptional regulator that plays a variety of roles in the immune system (1). For example, E4BP4/NFIL3 is required for bone marrow-derived NK cell development (2,3). In addition, it regulates IgE class switching in B cells by controlling IL-4 dependent induction of germ-line ε (GLε) transcription (4). In macrophages, E4BP4/NFIL3 is induced following exposure to bacteria and negatively regulates transcription of IL-12 p40. E4BP4/NFIL3 also controls cytokine production by T cells. Th2 cells from E4BP4/NFIL3(-/-) mice produced elevated levels of IL-5 an IL-13, but reduced levels of IL-4 ad IL-10 (5,6). Chronically stimulated Th1 cells, regulatory T cells, and NKT cells from E4BP4/NFIL3(-/-) mice all produce reduced levels of IL-10 and IL-13 (6). Finally, E4BP4/NFIL3 is also required for the development of conventional CD8α+ dendritic cells, as this subset is absent from E4BP4/NFIL3(-/-) mice (7).
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.