Render Target: STATIC
Render Timestamp: 2025-02-12T10:50:30.556Z
Commit: 7500bcdc731e9059bbdfbdbe9e72caa896e426e8
XML generation date: 2025-02-04 23:01:17.550
Product last modified at: 2025-02-05T09:00:10.112Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ECHS1 (F4A1Z) Rabbit mAb #18420

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 25
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ECHS1 (F4A1Z) Rabbit mAb recognizes endogenous levels of total ECHS1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ECHS1 protein.

    Background

    Mitochondrial enoyl-CoA hydratase short chain 1 (ECHS1), also known as short-chain enoyl-CoA hydratase (SCEH), is active in many metabolic pathways, including short-chain fatty acid β-oxidation and branched-chain amino acid catabolic pathway (1-3). Studies show that lysine catabolism in glioblastoma stem cells (GSCs) is altered via upregulation of lysine transporter SLC7A2 and crotonyl-CoA-producing enzyme GCDH and downregulation of ECHS1. These changes result in histone H4 lysine crotonylation. Lysine restriction decreases histone lysine crotonylation and reduces tumor growth (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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