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33437
ECM Profiling Sampler Kit
Primary Antibodies
Antibody Sampler Kit

ECM Profiling Sampler Kit #33437

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other Image 1 - ECM Profiling Sampler Kit

Immunoprecipitation of MMP-1 protein from SCaBER cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MMP-1 (E9S9N) Rabbit mAb. Western blot analysis was performed using MMP-1 (E9S9N) Rabbit mAb. Anti-rabbit IgG (light chain specific), HRP-linked Antibody was used as secondary antibody.

other Image 2 - ECM Profiling Sampler Kit

Confocal immunofluorescent analysis of U-87MG cells (left, positive) and HCT-15 cells (right, negative) using MMP-2 (D4M2N) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

other Image 3 - ECM Profiling Sampler Kit

Flow cytometric analysis of serum starved U-2 OS cells, untreated (blue) or treated with TPA (200nM, 24 hours) using MMP-9 (D6O3H) XP Rabbit mAb (green). Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) was used as a secondary antibody.

other Image 4 - ECM Profiling Sampler Kit

Western blot analysis of extracts from various cell lines using MMP-13 (E4W3T) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

other Image 5 - ECM Profiling Sampler Kit

Confocal immunofluorescent analysis of U-118 MG cells (left, positive) and PANC-1 cells (right, negative) using Fibronectin/FN1 (E5H6X) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 650 Phalloidin #12956 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

other Image 6 - ECM Profiling Sampler Kit

Western blot analysis of extracts from various cell lines using MMP-3 (D7F5B) Rabbit mAb.

other Image 7 - ECM Profiling Sampler Kit

Western blot analysis of extracts from various cell lines using LOX (D8F2K) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

other Image 8 - ECM Profiling Sampler Kit

Western blot analysis of extracts from various cell lines using COL11A1 (E6O7R) Rabbit mAb (upper), or

β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, A-204 cells have higher levels of COL11A1 expression as compared to the other cell lines shown.

other Image 9 - ECM Profiling Sampler Kit

Confocal immunofluorescent analysis of U-118 MG cells (left, positive) and HT-29 cells (right, negative) using COL1A1 (E6A8E) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

other Image 10 - ECM Profiling Sampler Kit

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

other Image 11 - ECM Profiling Sampler Kit

Western blot analysis of extracts from various cell lines using MMP-1 (E9S9N) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

other Image 12 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human bladder adenocarcinoma using MMP-2 (D4M2N) Rabbit mAb.

other Image 13 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb.

other Image 14 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human neuroendocrine carcinoma of the lung using Fibronectin/FN1 (E5H6X) Rabbit mAb performed on the Leica® BOND™ Rx.

other Image 15 - ECM Profiling Sampler Kit

Immunoprecipitation of COL1A1 protein from SK-N-SH cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is COL1A1 (E6A8E) Rabbit mAb. Western blot analysis was performed using COL1A1 (E6A8E) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as secondary antibody.

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other Image 16 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded U-87 MG cell pellet (left, positive) or HCT-15 cell pellet (right, negative) using MMP-2 (D4M2N) Rabbit mAb.

other Image 17 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb.

other Image 18 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded Hep G2 cell pellet (left, positive) or PANC-1 cell pellet (right, negative) using Fibronectin/FN1 (E5H6X) Rabbit mAb.

other Image 19 - ECM Profiling Sampler Kit

Western blot analysis of extracts from various cell lines using COL1A1 (E6A8E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

other Image 20 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using MMP-2 (D4M2N) Rabbit mAb.

other Image 21 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded U-2 OS cell pellets, untreated (left) or treated with TPA #4174 (right), using MMP-9 (D6O3H) XP® Rabbit mAb.

other Image 22 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.

other Image 23 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using MMP-2 (D4M2N) Rabbit mAb.

other Image 24 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

other Image 25 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.

other Image 26 - ECM Profiling Sampler Kit

Immunoprecipitation of MMP-2 protein from U-118MG cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MMP-2 (D4M2N) Rabbit mAb. Western blot analysis was performed using MMP-2 (D4M2N) Rabbit mAb as primary antibody. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as secondary antibody.

other Image 27 - ECM Profiling Sampler Kit

Western blot analysis of concentrated, serum-free cultured medium from U-2 OS cells, untreated (-) or treated with TPA #4174 (200 nM, 48 hr; +), using MMP-9 (D6O3H) XP® Rabbit mAb.

other Image 28 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human adenoid cystic carcinoma of the trachea using Fibronectin/FN1 (E5H6X) Rabbit mAb (left) or Fibronectin Rabbit mAb (right). These two antibodies detect independent, unique epitopes on human Fibronectin/FN1 protein. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.

other Image 29 - ECM Profiling Sampler Kit

Western blot analysis of extracts from various cell lines using MMP-2 (D4M2N) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

other Image 30 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

other Image 31 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.

other Image 32 - ECM Profiling Sampler Kit

Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.

other Image 33 - ECM Profiling Sampler Kit

Immunoprecipitation of Fibronectin/FN1 protein from Hep G2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Fibronectin/FN1 (E5H6X) Rabbit mAb. Western blot analysis was performed using Fibronectin/FN1 (E5H6X) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.

other Image 34 - ECM Profiling Sampler Kit

Western blot analysis of extracts from various cell lines using Fibronectin/FN1 (E5H6X) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The absence of Fibronectin/FN1 expression in PANC-1 cell extracts is consistent with molecular and proteomic expression profiling data, confirming specificity of the antibody.

To Purchase # 33437T
Product # Size Price
33437T
1 Kit  (9 x 20 µl) $ 609

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
MMP-1 (E9S9N) Rabbit mAb 54376 20 µl
  • WB
  • IP
H 55 Rabbit IgG
MMP-2 (D4M2N) Rabbit mAb 40994 20 µl
  • WB
  • IP
  • IHC
  • IF
H 64,72 Rabbit IgG
MMP-9 (D6O3H) XP® Rabbit mAb 13667 20 µl
  • WB
  • IHC
  • F
H 84, 92 Rabbit IgG
MMP-13 (E4W3T) Rabbit mAb 69926 20 µl
  • WB
H 60 Rabbit IgG
Fibronectin/FN1 (E5H6X) Rabbit mAb 26836 20 µl
  • WB
  • IP
  • IHC
  • IF
H 300 Rabbit IgG
MMP-3 (D7F5B) Rabbit mAb 14351 20 µl
  • WB
H R 60 Rabbit IgG
LOX (D8F2K) Rabbit mAb 58135 20 µl
  • WB
H M 54, 56 Rabbit IgG
COL11A1 (E6O7R) Rabbit mAb 70458 20 µl
  • WB
H 250 Rabbit IgG
COL1A1 (E6A8E) Rabbit mAb 39952 20 µl
  • WB
  • IP
  • IF
H 220 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The ECM Profiling Sampler Kit provides an economical means of detecting endogenous levels of the specific ECM components using the corresponding antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the ECM Profiling Sampler Kit detects endogenous levels of its target protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val267 of human MMP-1 protein, Phe542 of human MMP-9 protein, Ser417 of human MMP-3 protein, carboxyl terminal region of human MMP13 protein, Glu320 of human COL11A1 protein, Lys170 of human COL1A1 protein, recombinant protein specific to the carboxy terminus of human MMP-2 protein, recombinant protein specific to the carboxy terminus of human FN1 protein, and a recombinant protein specific to human LOX protein.

Background

The extracellular matrix (ECM) is a cell surface associated three-dimensional macromolecular network composed of collagens, proteoglycans/glycosaminoglycans, elastin, fibronectin, laminins, and several other glycoproteins (1). The network provides a dynamic microenvironment surrounding the cell enabling it to carry on its function. Among the ECM proteins, fibronectin functions as a mediator to bridge distinct ECM components such as collagens, growth factors, as well as cell surface integrins to regulate ECM structural change and initiate signaling pathways (2). During normal development and pathological conditions, the ECM network is highly dynamic and continuously undergoes remodeling marked by the change of the ECM structural components, such as COL1A1, COL11A1, fibronectin, and versican. The matrix-degrading enzyme MMPs such as MMP1, MMP2, and MMP9 are highly involved in this process (4). Additional players in this process are the LOX family members of lysyl oxidase. They catalyze the first step of the covalent cross-linking of ECM proteins, collagens, and elastin, which contributes to ECM stiffness and mechanical properties (5).

  1. Theocharis, A.D. et al. (2019) FEBS J 286, 2830-69.
  2. Zollinger, A.J. and Smith, M.L. (2017) Matrix Biol 60-61, 27-37.
  3. Paolillo, M. and Schinelli, S. (2019) Int J Mol Sci 20, pii: E4947. doi: 10.3390/ijms20194947.
  4. Bonnans, C. et al. (2014) Nat Rev Mol Cell Biol 15, 786-801.
  5. Amendola, P.G. et al. (2019) Cancers (Basel) 11, pii: E729. doi: 10.3390/cancers11050729.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

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