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33437
ECM Profiling Sampler Kit
Primary Antibodies
Antibody Sampler Kit

ECM Profiling Sampler Kit #33437

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ECM Profiling Sampler Kit: Image 1

Immunoprecipitation of MMP-1 protein from SCaBER cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MMP-1 (E9S9N) Rabbit mAb. Western blot analysis was performed using MMP-1 (E9S9N) Rabbit mAb. Anti-rabbit IgG (light chain specific), HRP-linked Antibody was used as secondary antibody.

ECM Profiling Sampler Kit: Image 2

Confocal immunofluorescent analysis of U-87MG cells (left, positive) and HCT-15 cells (right, negative) using MMP-2 (D4M2N) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

ECM Profiling Sampler Kit: Image 3

Flow cytometric analysis of serum starved U-2 OS cells, untreated (blue) or treated with TPA (200nM, 24 hours) using MMP-9 (D6O3H) XP Rabbit mAb (green). Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) was used as a secondary antibody.

ECM Profiling Sampler Kit: Image 4

Western blot analysis of extracts from various cell lines using MMP-13 (E4W3T) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

ECM Profiling Sampler Kit: Image 5

Confocal immunofluorescent analysis of U-118 MG cells (left, positive) and PANC-1 cells (right, negative) using Fibronectin/FN1 (E5H6X) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 650 Phalloidin #12956 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

ECM Profiling Sampler Kit: Image 6

Western blot analysis of extracts from various cell lines using MMP-3 (D7F5B) Rabbit mAb.

ECM Profiling Sampler Kit: Image 7

Western blot analysis of extracts from various cell lines using LOX (D8F2K) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

ECM Profiling Sampler Kit: Image 8

Western blot analysis of extracts from various cell lines using COL11A1 (E6O7R) Rabbit mAb (upper), or

β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, A-204 cells have higher levels of COL11A1 expression as compared to the other cell lines shown.

ECM Profiling Sampler Kit: Image 9

Confocal immunofluorescent analysis of U-118 MG cells (left, positive) and HT-29 cells (right, negative) using COL1A1 (E6A8E) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

ECM Profiling Sampler Kit: Image 10

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

ECM Profiling Sampler Kit: Image 11

Western blot analysis of extracts from various cell lines using MMP-1 (E9S9N) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

ECM Profiling Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded human bladder adenocarcinoma using MMP-2 (D4M2N) Rabbit mAb.

ECM Profiling Sampler Kit: Image 13

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb.

ECM Profiling Sampler Kit: Image 14

Immunohistochemical analysis of paraffin-embedded human neuroendocrine carcinoma of the lung using Fibronectin/FN1 (E5H6X) Rabbit mAb performed on the Leica® BOND™ Rx.

ECM Profiling Sampler Kit: Image 15

Immunoprecipitation of COL1A1 protein from SK-N-SH cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is COL1A1 (E6A8E) Rabbit mAb. Western blot analysis was performed using COL1A1 (E6A8E) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as secondary antibody.

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ECM Profiling Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded U-87 MG cell pellet (left, positive) or HCT-15 cell pellet (right, negative) using MMP-2 (D4M2N) Rabbit mAb.

ECM Profiling Sampler Kit: Image 17

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb.

ECM Profiling Sampler Kit: Image 18

Immunohistochemical analysis of paraffin-embedded Hep G2 cell pellet (left, positive) or PANC-1 cell pellet (right, negative) using Fibronectin/FN1 (E5H6X) Rabbit mAb.

ECM Profiling Sampler Kit: Image 19

Western blot analysis of extracts from various cell lines using COL1A1 (E6A8E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

ECM Profiling Sampler Kit: Image 20

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using MMP-2 (D4M2N) Rabbit mAb.

ECM Profiling Sampler Kit: Image 21

Immunohistochemical analysis of paraffin-embedded U-2 OS cell pellets, untreated (left) or treated with TPA #4174 (right), using MMP-9 (D6O3H) XP® Rabbit mAb.

ECM Profiling Sampler Kit: Image 22

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.

ECM Profiling Sampler Kit: Image 23

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using MMP-2 (D4M2N) Rabbit mAb.

ECM Profiling Sampler Kit: Image 24

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

ECM Profiling Sampler Kit: Image 25

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.

ECM Profiling Sampler Kit: Image 26

Immunoprecipitation of MMP-2 protein from U-118MG cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MMP-2 (D4M2N) Rabbit mAb. Western blot analysis was performed using MMP-2 (D4M2N) Rabbit mAb as primary antibody. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as secondary antibody.

ECM Profiling Sampler Kit: Image 27

Western blot analysis of concentrated, serum-free cultured medium from U-2 OS cells, untreated (-) or treated with TPA #4174 (200 nM, 48 hr; +), using MMP-9 (D6O3H) XP® Rabbit mAb.

ECM Profiling Sampler Kit: Image 28

Immunohistochemical analysis of paraffin-embedded human adenoid cystic carcinoma of the trachea using Fibronectin/FN1 (E5H6X) Rabbit mAb (left) or Fibronectin Rabbit mAb (right). These two antibodies detect independent, unique epitopes on human Fibronectin/FN1 protein. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.

ECM Profiling Sampler Kit: Image 29

Western blot analysis of extracts from various cell lines using MMP-2 (D4M2N) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

ECM Profiling Sampler Kit: Image 30

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

ECM Profiling Sampler Kit: Image 31

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.

ECM Profiling Sampler Kit: Image 32

Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin's lymphoma using Fibronectin/FN1 (E5H6X) Rabbit mAb.

ECM Profiling Sampler Kit: Image 33

Immunoprecipitation of Fibronectin/FN1 protein from Hep G2 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Fibronectin/FN1 (E5H6X) Rabbit mAb. Western blot analysis was performed using Fibronectin/FN1 (E5H6X) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.

ECM Profiling Sampler Kit: Image 34

Western blot analysis of extracts from various cell lines using Fibronectin/FN1 (E5H6X) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The absence of Fibronectin/FN1 expression in PANC-1 cell extracts is consistent with molecular and proteomic expression profiling data, confirming specificity of the antibody.

To Purchase # 33437T
Product # Size Price
33437T
1 Kit  (9 x 20 µl) $ 609

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
MMP-1 (E9S9N) Rabbit mAb 54376 20 µl
  • WB
  • IP
H 55 Rabbit IgG
MMP-2 (D4M2N) Rabbit mAb 40994 20 µl
  • WB
  • IP
  • IHC
  • IF
H 64,72 Rabbit IgG
MMP-9 (D6O3H) XP® Rabbit mAb 13667 20 µl
  • WB
  • IHC
  • F
H 84, 92 Rabbit IgG
MMP-13 (E4W3T) Rabbit mAb 69926 20 µl
  • WB
H 60 Rabbit IgG
Fibronectin/FN1 (E5H6X) Rabbit mAb 26836 20 µl
  • WB
  • IP
  • IHC
  • IF
H 300 Rabbit IgG
MMP-3 (D7F5B) Rabbit mAb 14351 20 µl
  • WB
H R 60 Rabbit IgG
LOX (D8F2K) Rabbit mAb 58135 20 µl
  • WB
H M 54, 56 Rabbit IgG
COL11A1 (E6O7R) Rabbit mAb 70458 20 µl
  • WB
H 250 Rabbit IgG
COL1A1 (E6A8E) Rabbit mAb 39952 20 µl
  • WB
  • IP
  • IF
H 220 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The ECM Profiling Sampler Kit provides an economical means of detecting endogenous levels of the specific ECM components using the corresponding antibodies. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the ECM Profiling Sampler Kit detects endogenous levels of its target protein.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val267 of human MMP-1 protein, Phe542 of human MMP-9 protein, Ser417 of human MMP-3 protein, carboxyl terminal region of human MMP13 protein, Glu320 of human COL11A1 protein, Lys170 of human COL1A1 protein, recombinant protein specific to the carboxy terminus of human MMP-2 protein, recombinant protein specific to the carboxy terminus of human FN1 protein, and a recombinant protein specific to human LOX protein.

Background

The extracellular matrix (ECM) is a cell surface associated three-dimensional macromolecular network composed of collagens, proteoglycans/glycosaminoglycans, elastin, fibronectin, laminins, and several other glycoproteins (1). The network provides a dynamic microenvironment surrounding the cell enabling it to carry on its function. Among the ECM proteins, fibronectin functions as a mediator to bridge distinct ECM components such as collagens, growth factors, as well as cell surface integrins to regulate ECM structural change and initiate signaling pathways (2). During normal development and pathological conditions, the ECM network is highly dynamic and continuously undergoes remodeling marked by the change of the ECM structural components, such as COL1A1, COL11A1, fibronectin, and versican. The matrix-degrading enzyme MMPs such as MMP1, MMP2, and MMP9 are highly involved in this process (4). Additional players in this process are the LOX family members of lysyl oxidase. They catalyze the first step of the covalent cross-linking of ECM proteins, collagens, and elastin, which contributes to ECM stiffness and mechanical properties (5).

  1. Theocharis, A.D. et al. (2019) FEBS J 286, 2830-69.
  2. Zollinger, A.J. and Smith, M.L. (2017) Matrix Biol 60-61, 27-37.
  3. Paolillo, M. and Schinelli, S. (2019) Int J Mol Sci 20, pii: E4947. doi: 10.3390/ijms20194947.
  4. Bonnans, C. et al. (2014) Nat Rev Mol Cell Biol 15, 786-801.
  5. Amendola, P.G. et al. (2019) Cancers (Basel) 11, pii: E729. doi: 10.3390/cancers11050729.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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